ArticleThe Journal of investigative dermatology2015
Keratinocyte Microvesicles Regulate the Expression of Multiple Genes in Dermal Fibroblasts.
Article in The Journal of investigative dermatology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 86 citations in OpenAlex.
- Interplay Between Keratinocytes and Fibroblasts: A Systematic Review Providing a New Angle for Understanding Skin Fibrotic Disorders.Frontiers in immunology · 2020Pooled it
- Purification of Extracellular Vesicles Secreted by Skin Fibroblasts and Keratinocytes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Exosomes in Skin of Color: Dermatological Insights, Therapeutic Potential, and Clinical Realities in India.Cureus · 2026Review
- The Therapeutic Potential of Extracellular Vesicles in Psoriasis Treatment: Mechanisms, Applications, and Prospects.International journal of molecular sciences · 2025Review
- GelMA hydrogel-loaded extracellular vesicles derived from keratinocytes promote skin microvasculature regeneration and wound healing in diabetic mice through activation of the PDGF-induced PI3K/AKT pathway.Cell biology and toxicology · 2025Article
- Exosomes: A Comprehensive Review for the Practicing Dermatologist.The Journal of clinical and aesthetic dermatology · 2025Review
- Epigenetics in Skin Homeostasis and Ageing.Epigenomes · 2025Review
- Roles for Exosomes in the Pathogenesis, Drug Delivery and Therapy of Psoriasis.Pharmaceutics · 2025Review
- Recent advances in the role of neuroregulation in skin wound healing.Burns & trauma · 2025Review
- The Central Roles of Keratinocytes in Coordinating Skin Immunity.The Journal of investigative dermatology · 2024Review
- Keratinocyte derived extracellular vesicles mediated crosstalk between epidermis and dermis in UVB-induced skin inflammation.Cell communication and signaling : CCS · 2024Article
- Article
- Extracellular Vesicles-Based Cell-Cell Communication in Melanoma: New Perspectives in Diagnostics and Therapy.International journal of molecular sciences · 2023Review
- Gingival epithelial cell-derived microvesicles activate mineralization in gingival fibroblasts.Scientific reports · 2022Article
- Photobiomodulation induces microvesicle release in human keratinocytes: PI3 kinase-dependent pathway role.Lasers in medical science · 2022Article
- Roles of MicroRNA-21 in Skin Wound Healing: A Comprehensive Review.Frontiers in pharmacology · 2022Review
- Skin cell-derived extracellular vesicles: a promising therapeutic strategy for cutaneous injury.Burns & trauma · 2022Review
- Native and engineered extracellular vesicles for wound healing.Frontiers in bioengineering and biotechnology · 2022Review
- Extracellular Vesicles in Skin Wound Healing.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Extracellular vesicles released from cells regulate many normal and pathological conditions. Little is known about the role of epidermal keratinocyte microvesicles (KC-MVs) in epithelial-stromal interaction that is essential for wound healing. We investigated, therefore, whether MV-like structures are present in human wounds and whether they affect wound healing-associated gene expression in dermal fibroblasts. In human wounds, MV-like vesicles were observed during active epithelial migration and early granulation tissue formation. When KC-MVs derived from keratinocyte-like cells (HaCaT) were added to fibroblast cultures, expression of 21 genes was significantly regulated (P<0.05) out of 80 genes investigated, including matrix metalloproteinase-1 and -3, interleukin-6 and -8, and genes associated with transforming growth factor-β signaling. Similar changes were observed at the protein level. MVs from normal epidermal keratinocytes showed similar response to HaCaT cells. KC-MVs activated ERK1/2, JNK, Smad, and p38 signaling pathways in fibroblasts with ERK1/2 signaling having the most prominent role in the MV-induced gene expression changes. KC-MVs stimulated fibroblast migration and induced fibroblast-mediated endothelial tube formation but did not affect collagen gel contraction by fibroblasts. The results demonstrate that keratinocyte microvesicles have a strong and a specific regulatory effect on fibroblasts that may modulate several aspects of wound healing.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.