ArticleBioconjugate chemistry2015
Synthesis and Incorporation of Unnatural Amino Acids To Probe and Optimize Protein Bioconjugations.
Article in Bioconjugate chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Supramolecular coiled-coil peptide platform for site-specific antibody drug conjugate engineering.Nature communications · 2026Article
- Development of programmable RNA imaging with RNA-guided GFP via click chemistry.Nucleic acids research · 2025Article
- A Supramolecular Self-assembly Approach to Site-Specific Antibody ConjugatesbioRxiv : the preprint server for biology · 2025Article
- Cracking the Code: Reprogramming the Genetic Script in Prokaryotes and Eukaryotes to Harness the Power of Noncanonical Amino Acids.Chemical reviews · 2024Review
- Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates.Science advances · 2020Article
- Double Nitroxide Labeling by Copper-Catalyzed Azide-Alkyne Cycloadditions with Noncanonical Amino Acids for Electron Paramagnetic Resonance Spectroscopy.ACS chemical biology · 2019Article
- Playing with the Molecules of Life.ACS chemical biology · 2018Review
- Practical Considerations, Challenges, and Limitations of Bioconjugation via Azide-Alkyne Cycloaddition.Bioconjugate chemistry · 2018Review
- Development of optimized conditions for Glaser-Hay bioconjugations.Bioorganic chemistry · 2018Article
- Investigation of copper-free alkyne/azide 1,3-dipolar cycloadditions using microwave irradiation.Bioorganic & medicinal chemistry letters · 2018Article
- Utilization of alkyne bioconjugations to modulate protein function.Bioorganic & medicinal chemistry letters · 2017Article
- Synthesis and incorporation of a caged tyrosine amino acid possessing a bioorthogonal handle.Tetrahedron letters · 2016Article
- Exploiting the co-reliance of tumours upon transport of amino acids and lactate: Gln and Tyr conjugates of MCT1 inhibitors.MedChemComm · 2016Article
- Azidoethoxyphenylalanine as a Vibrational Reporter and Click Chemistry Partner in Proteins.Chemistry (Weinheim an der Bergstrasse, Germany) · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The utilization of unnatural amino acids (UAAs) in bioconjugations is ideal due to their ability to confer a degree of bioorthogonality and specificity. In order to elucidate optimal conditions for the preparation of bioconjugates with UAAs, we synthesized 9 UAAs with variable methylene tethers (2-4) and either an azide, alkyne, or halide functional group. All 9 UAAs were then incorporated into green fluorescent protein (GFP) using a promiscuous aminoacyl-tRNA synthetase. The different bioconjugations were then analyzed for optimal tether length via reaction with either a fluorophore or a derivatized resin. Interestingly, the optimal tether length was found to be dependent on the type of reaction. Overall, these findings provide a better understanding of various parameters that can be optimized for the efficient preparation of bioconjugates.
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Registered trials
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