ArticleBiophysical journal2015
Feeling for Filaments: Quantification of the Cortical Actin Web in Live Vascular Endothelium.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- The membrane-to-cortex distance regulates mDia1 activity to control cortical mechanics.Nature communications · 2026Article
- Quantifying cytoskeletal organization from optical microscopy data.Frontiers in cell and developmental biology · 2023Review
- Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy.Progress in biophysics and molecular biology · 2022Review
- A cortactin CTTN coding SNP contributes to lung vascular permeability and inflammatory disease severity in African descent subjects.Translational research : the journal of laboratory and clinical medicine · 2022Article
- Rapid shear stress-dependent ENaC membrane insertion is mediated by the endothelial glycocalyx and the mineralocorticoid receptor.Cellular and molecular life sciences : CMLS · 2022Article
- Visualizing intracellular nanostructures of living cells by nanoendoscopy-AFM.Science advances · 2021Article
- Biomechanical properties of endothelial glycocalyx: An imperfect pendulum.Matrix biology plus · 2021Article
- Evaluation of the In Vitro Biocompatibility of PEDOT:Nafion Coatings.Nanomaterials (Basel, Switzerland) · 2021Article
- Article
- Subsurface Imaging of Cell Organelles by Force Microscopy.ACS nano · 2019Article
- Anisotropy vs isotropy in living cell indentation with AFM.Scientific reports · 2019Article
- Endothelial Nanomechanics in the Context of Endothelial (Dys)function and Inflammation.Antioxidants & redox signaling · 2019Review
- Interrogating Synaptic Architecture: Approaches for Labeling Organelles and Cytoskeleton Components.Frontiers in synaptic neuroscience · 2019Review
- Mapping heterogeneity of cellular mechanics by multi-harmonic atomic force microscopy.Nature protocols · 2018Article
- The actin cortex at a glance.Journal of cell science · 2018Review
- Quantitative observations on cytoskeleton changes of osteocytes at different cell parts using digital holographic microscopy.Biomedical optics express · 2018Article
- Cortical actin contributes to spatial organization of ER-PM junctions.Molecular biology of the cell · 2017Article
- Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells.Scientific reports · 2017Article
- Intracellular nanoparticle dynamics affected by cytoskeletal integrity.Soft matter · 2017Article
- Glycerol-3-phosphate acyltransferase 2 expression modulates cell roughness and membrane permeability: An atomic force microscopy study.PloS one · 2017Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Contact-mode atomic force microscopy (AFM) has been shown to reveal cortical actin structures. Using live endothelial cells, we visualized cortical actin dynamics simultaneously by AFM and confocal fluorescence microscopy. We present a method that quantifies dynamic changes in the mechanical ultrastructure of the cortical actin web. We argue that the commonly used, so-called error signal imaging in AFM allows a qualitative, but not quantitative, analysis of cortical actin dynamics. The approach we used comprises fast force-curve-based topography imaging and subsequent image processing that enhances local height differences. Dynamic changes in the organization of the cytoskeleton network can be observed and quantified by surface roughness calculations and automated morphometrics. Upon treatment with low concentrations of the actin-destabilizing agent cytochalasin D, the cortical cytoskeleton network is thinned out and the average mesh size increases. In contrast, jasplakinolide, a drug that enhances actin polymerization, consolidates the cytoskeleton network and reduces the average mesh area. In conclusion, cortical actin dynamics can be quantified in live cells. To our knowledge, this opens a new pathway for conducting quantitative structure-function analyses of the endothelial actin web just beneath the apical plasma membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.