ArticleScientific reports2015
Cultivating the uncultured: growing the recalcitrant cluster-2 Frankia strains.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 91 citations in OpenAlex.
- Integrated multi-omics decipher the complex nodule microbiota and distinct Frankiaceae symbiotic traits in wild actinorhizal plants.The New phytologist · 2026Article
- Host-dependent specialized metabolism of nitrogen export in actinorhizal nodules induced by diazotrophic Actinomycetota Frankia cluster-2.Journal of experimental botany · 2025Article
- Article
- A root nodule microbiome sequencing data set from red alder (Alnus rubra Bong.).Scientific data · 2024Article
- Frankia [NiFe] uptake hydrogenases and genome reduction: different lineages of loss.FEMS microbiology ecology · 2024Article
- Root nodules of red alder (Alnus rubra) and sitka alder (Alnus viridis ssp. sinuata) are inhabited by taxonomically diverse cultivable microbial endophytes.MicrobiologyOpen · 2024Article
- Genome analysis and biogeographic distribution of the earliest divergent Frankia clade in the southern hemisphere.FEMS microbiology ecology · 2024Article
- MAGs-centric crack: how long will, spore-positiveFrontiers in microbiology · 2024Article
- Draft Genomes ofJournal of genomics · 2023Article
- A tale of two lineages: how the strains of the earliest divergent symbiotic Frankia clade spread over the world.BMC genomics · 2022Article
- Pseudogenization of the rhizobium-responsive EXOPOLYSACCHARIDE RECEPTOR in Parasponia is a rare event in nodulating plants.BMC plant biology · 2022Article
- Taxogenomic status of phylogenetically distantFrontiers in microbiology · 2022Article
- Decrypting phytomicrobiome of the neurotoxic actinorhizal species,Frontiers in microbiology · 2022Article
- Root Nodule Microsymbionts of NativeMicrobiology insights · 2022Article
- Draft Genomes of SymbioticJournal of genomics · 2022Article
- Draft Genomes of Nitrogen-fixingJournal of genomics · 2022Article
- The Peptidoglycan Biosynthesis GeneGenes · 2020Article
- Frankia-Enriched Metagenomes from the Earliest Diverging Symbiotic Frankia Cluster: They Come in Teams.Genome biology and evolution · 2019Article
- Draft genome sequences for three unisolatedJournal of genomics · 2019Article
- Genomic Insights Into Plant-Growth-Promoting Potentialities of the GenusFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The repeated failures reported in cultivating some microbial lineages are a major challenge in microbial ecology and probably linked, in the case of Frankia microsymbionts to atypical patterns of auxotrophy. Comparative genomics of the so far uncultured cluster-2 Candidatus Frankia datiscae Dg1, with cultivated Frankiae has revealed genome reduction, but no obvious physiological impairments. A direct physiological assay on nodule tissues from Coriaria myrtifolia infected with a closely-related strain permitted the identification of a requirement for alkaline conditions. A high pH growth medium permitted the recovery of a slow-growing actinobacterium. The strain obtained, called BMG5.1, has short hyphae, produced diazovesicles in nitrogen-free media, and fulfilled Koch's postulates by inducing effective nodules on axenically grown Coriaria spp. and Datisca glomerata. Analysis of the draft genome confirmed its close proximity to the Candidatus Frankia datiscae Dg1 genome with the absence of 38 genes (trehalose synthase, fumarylacetoacetase, etc) in BMG5.1 and the presence of 77 other genes (CRISPR, lanthionine synthase, glutathione synthetase, catalase, Na+/H+ antiporter, etc) not found in Dg1. A multi-gene phylogeny placed the two cluster-2 strains together at the root of the Frankia radiation.
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