ArticleSensors (Basel, Switzerland)2015
Designing a Microfluidic Device with Integrated Ratiometric Oxygen Sensors for the Long-Term Control and Monitoring of Chronic and Cyclic Hypoxia.
Article in Sensors (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Smart microdevices for biomedical drug delivery: endogenous stimuli as the key to safer therapeutics.RSC advances · 2026Review
- Post-myocardial Infarction Cardiac Remodeling: Multidimensional Mechanisms and Clinical Prospects of Stem Cell Therapy.Stem cell reviews and reports · 2025Review
- Improving tumor microenvironment assessment in chip systems through next-generation technology integration.Frontiers in bioengineering and biotechnology · 2024Review
- Microfluidic integrated gas sensors for smart analyte detection: a comprehensive review.Frontiers in chemistry · 2023Review
- Metabolic Switching of Tumor Cells under Hypoxic Conditions in a Tumor-on-a-chip Model.Micromachines · 2020Article
- Microfluidic platform for three-dimensional cell culture under spatiotemporal heterogeneity of oxygen tension.APL bioengineering · 2020Article
- Long-term monitoring in a microfluidic system to study tumour spheroid response to chronic and cycling hypoxia.Scientific reports · 2019Article
- Spatial control of oxygen delivery to three-dimensional cultures alters cancer cell growth and gene expression.Journal of cellular physiology · 2019Article
- Measuring and regulating oxygen levels in microphysiological systems: design, material, and sensor considerations.The Analyst · 2019Review
- A three-dimensional engineered heterogeneous tumor model for assessing cellular environment and response.Nature protocols · 2018Article
- In vitro microfluidic models of tumor microenvironment to screen transport of drugs and nanoparticles.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2017Review
- Microfluidic Platform for the Long-Term On-Chip Cultivation of Mammalian Cells for Lab-On-A-Chip Applications.Sensors (Basel, Switzerland) · 2017Article
- The ascendance of microphysiological systems to solve the drug testing dilemma.Future science OA · 2017Review
- Microchambers with Solid-State Phosphorescent Sensor for Measuring Single Mitochondrial Respiration.Sensors (Basel, Switzerland) · 2016Article
- On-chip clearing of arrays of 3-D cell cultures and micro-tissues.Biomicrofluidics · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Control of oxygen over cell cultures in vitro is a topic of considerable interest, as chronic and cyclic hypoxia can alter cell behaviour. Both static and transient hypoxic levels have been found to affect tumour cell behaviour; it is potentially valuable to include these effects in early, in vitro stages of drug screening. A barrier to their inclusion is that rates of transient hypoxia can be a few cycles/hour, which is difficult to reproduce in traditional in vitro cell culture environments due to long diffusion distances from control gases to the cells. We use a gas-permeable three-layer microfluidic device to achieve spatial and temporal oxygen control with biologically-relevant switching times. We measure the oxygen profiles with integrated, ratiometric optical oxygen sensors, demonstrate sensor and system stability over multi-day experiments, and characterize a pre-bleaching process to improve sensor stability. We show, with both finite-element modelling and experimental data, excellent control over the oxygen levels by the device, independent of fluid flow rate and oxygenation for the operating flow regime. We measure equilibration times of approximately 10 min, generate complex, time-varying oxygen profiles, and study the effects of oxygenated media flow rates on the measured oxygen levels. This device could form a useful tool for future long-term studies of cell behaviour under hypoxia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.