ArticleBMC genetics2015
Assessment of genetic diversity in Ethiopian field pea (Pisum sativum L.) accessions with newly developed EST-SSR markers.
Article in BMC genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Scaling field pea technology in dryland areas through a cluster approach: The case of Waghimra zone, Ethiopia.PloS one · 2026Article
- RNA-Seq-based transcriptomics reveals differential gene expression between two Pisum sativum subspecies and uncovers their molecular marker profiles.BMC genomics · 2025Article
- Genetic Diversity and Population Structure of Shanlan Upland Rice Germplasm Based on SSR Markers.Plants (Basel, Switzerland) · 2025Article
- SeqSNP-Based Targeted GBS Provides Insight into the Genetic Relationships among Global Collections ofGenes · 2024Article
- Genome-wide identification and expression analysis of the SPL transcription factor family and its response to abiotic stress in Pisum sativum L.BMC genomics · 2024Article
- Genetic Diversity of Durum Wheat (Genes · 2023Article
- Analysis of Genetic Diversity of Fescue Populations from the Highlands of Bolivia Using EST-SSR Markers.Genes · 2022Article
- Article
- Genomics Enabled Breeding Strategies for Major Biotic Stresses in Pea (Frontiers in plant science · 2022Review
- RNA-Seq Provides Novel Genomic Resources for Noug (Frontiers in plant science · 2022Article
- Article
- Novel Expressed Sequence Tag-Derived and Other Genomic Simple Sequence Repeat Markers Revealed Genetic Diversity in Ethiopian Finger Millet Landrace Populations and Cultivars.Frontiers in plant science · 2021Article
- New Transcriptome-Based SNP Markers for Noug (Genes · 2020Article
- Genetic diversity of avocado from the southern highlands of Tanzania as revealed by microsatellite markers.Hereditas · 2020Article
- Identification of Differentially Expressed Genes Involved in the Molecular Mechanism of Pericarp Elongation and Differences in Sucrose and Starch Accumulation between Vegetable and Grain Pea (International journal of molecular sciences · 2019Article
- Comparative Transcriptome Analysis Identified Candidate Genes Related to Bailinggu Mushroom Formation and Genetic Markers for Genetic Analyses and Breeding.Scientific reports · 2017Article
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4 authors.
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Abstract
backgroundField pea (Pisum sativum L.) is among the prominent crops in the world as food and feed. There are relatively few simple sequence repeat (SSR) markers developed from expressed sequence tags (ESTs) in P. sativum.
resultsIn the present study, 15 new EST-SSR markers were developed from publicly available ESTs. These markers have successfully amplified their target loci across seven Pisum sativum subsp. sativum accessions. Eleven (73%) of these SSRs were trinucleotide repeats, two (13%) dinucleotide and two (13%) were hexanucleotide repeats. Across-taxa transferability of these new markers was also tested on other subspecies of Pisum as well as on P. fulvum, Vicia faba and Lens culinaris. In Pisum sativum subsp. sativum, 13 of the 15 markers were polymorphic and 12 of them subsequently used for genetic diversity analysis. Forty six accessions, of which 43 were from Ethiopia, were subjected to genetic diversity analysis using these newly developed markers. All accessions were represented by 12 individuals except two (NGB103816 and 237508) that were represented by 9 and 11 individuals, respectively. A total of 37 alleles were detected across all accessions. PS10 was the most polymorphic locus with six alleles, and the average number of alleles per locus over the 12 polymorphic loci was 3.1. Several rare and private alleles were also revealed. The most distinct accession (32048) had private alleles at three loci with 100% frequency.
conclusionThese newly developed EST-SSR primer-pairs successfully amplified expected loci in P. sativum subsp. sativum as well as in other subspecies of the genus Pisum and related genera. High levels of genetic variation were detected in field pea accessions from Ethiopia using these markers. This result implies the potential of the Ethiopian field pea gene pool for improvement of field peas in various desirable traits. In addition, these markers could be a valuable asset in resolving the inconsistency in the taxonomic status of the different subspecies of genus Pisum as well as for characterization of field pea accessions in different gene banks around the world for breeding and conservation purposes.
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