Evidence map›Paper›PMID 26286536›Full record

ArticleThe Plant cell2015

Lost in Translation: Pitfalls in Deciphering Plant Alternative Splicing Transcripts.

John W S Brown, Craig G Simpson, Yamile Marquez, Geoffrey M Gadd, Andrea Barta, Maria Kalyna

Abstract read
In one paragraph

Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

John W S BrownPlant Sciences Division, School of Life Sciences, University of Dundee, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom j.w.s.brown@dundee.ac.uk.ORCID http://orcid.org/0000-0003-2979-779X
Craig G SimpsonCell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.ORCID http://orcid.org/0000-0002-1723-1492
Yamile MarquezMax F. Perutz Laboratories, Medical University of Vienna, 1030 Vienna, Austria Center for Integrative Bioinformatics Vienna, Max F. Perutz Laboratories, Medical University of Vienna, 1030 Vienna, Austria.ORCID http://orcid.org/0000-0003-1686-5992
Geoffrey M GaddGeomicrobiology Group, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom.ORCID http://orcid.org/0000-0001-6874-870X
Andrea BartaMax F. Perutz Laboratories, Medical University of Vienna, 1030 Vienna, Austria.ORCID http://orcid.org/0000-0002-8851-406X
Maria KalynaDepartment of Applied Genetics and Cell Biology, BOKU-University of Natural Resources and Life Sciences, 1190 Vienna, Austria.ORCID http://orcid.org/0000-0003-4702-7625

Funding

Austrian Science Fund FWF P 26333Biotechnology and Biological Sciences Research Council BB/K006568/1
6 · The paper itself

Abstract

Transcript annotation in plant databases is incomplete and often inaccurate, leading to misinterpretation. As more and more RNA-seq data are generated, plant scientists need to be aware of potential pitfalls and understand the nature and impact of specific alternative splicing transcripts on protein production. A primary area of concern and the topic of this article is the (mis)annotation of open reading frames and premature termination codons. The basic message is that to adequately address expression and functions of transcript isoforms, it is necessary to be able to predict their fate in terms of whether protein isoforms are generated or specific transcripts are unproductive or degraded.

Indexed as

Alternative SplicingModels, GeneticOpen Reading FramesPlant ProteinsPlantsProtein BiosynthesisProtein IsoformsRNA, MessengerRNA StabilityPlant ProteinsProtein IsoformsRNA, Messenger

Identifiers

PMID26286536
PMCPMC4568512

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.