ArticleScientific reports2015
Long- and Short-Range Electrostatic Fields in GFP Mutants: Implications for Spectral Tuning.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence protein.Journal of advanced research · 2026Article
- Article
- Impact of the Protein Environment on Two-Photon Absorption Cross-Sections of the GFP Chromophore Anion Resolved at the XMCQDPT2 Level of Theory.International journal of molecular sciences · 2023Article
- Complexation of Green and Red Kaede Fluorescent Protein Chromophores by a Zwitterion to Probe Electrostatic and Induction Field Effects.The journal of physical chemistry. A · 2022Article
- Energy, Structures, and Response Properties with a Fully Coupled QM/AMOEBA/ddCOSMO Implementation.Journal of chemical theory and computation · 2021Article
- Local Electric Field Controls Fluorescence Quantum Yield of Red and Far-Red Fluorescent Proteins.Frontiers in molecular biosciences · 2021Article
- Mechanism of Color and Photoacidity Tuning for the Protonated Green Fluorescent Protein Chromophore.Journal of the American Chemical Society · 2020Article
- Structure-fluorescence activation relationships of a large Stokes shift fluorogenic RNA aptamer.Nucleic acids research · 2019Article
- Unified Model for Photophysical and Electro-Optical Properties of Green Fluorescent Proteins.Journal of the American Chemical Society · 2019Article
- Understanding the Fluorescence Change in Red Genetically Encoded Calcium Ion Indicators.Biophysical journal · 2019Article
- Article
- Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation.The journal of physical chemistry letters · 2017Article
- Mechanism and bottlenecks in strand photodissociation of split green fluorescent proteins (GFPs).Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Deciphering the molecular mechanism responsible for GCaMP6m's Ca2+-dependent change in fluorescence.PloS one · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The majority of protein functions are governed by their internal local electrostatics. Quantitative information about these interactions can shed light on how proteins work and allow for improving/altering their performance. Green fluorescent protein (GFP) and its mutation variants provide unique optical windows for interrogation of internal electric fields, thanks to the intrinsic fluorophore group formed inside them. Here we use an all-optical method, based on the independent measurements of transition frequency and one- and two-photon absorption cross sections in a number of GFP mutants to evaluate these internal electric fields. Two physical models based on the quadratic Stark effect, either with or without taking into account structural (bond-length) changes of the chromophore in varying field, allow us to separately evaluate the long-range and the total effective (short- and long-range) fields. Both types of the field quantitatively agree with the results of independent molecular dynamic simulations, justifying our method of measurement.
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