ArticleScientific reports2015
Alpha-ring Independent Assembly of the 20S Proteasome.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Plasticity in the structure and assembly of proteasomes.The Journal of biological chemistry · 2026Review
- Hsp70 and Hsp110 Chaperones Promote Early Steps of Proteasome Assembly.Biomolecules · 2022Article
- Understanding the separation of timescales in bacterial proteasome core particle assembly.Biophysical journal · 2022Article
- Upregulated Proteasome Subunits in COVID-19 Patients: A Link with Hypoxemia, Lymphopenia and Inflammation.Biomolecules · 2022Article
- An evolutionarily distinct chaperone promotes 20S proteasome α-ring assembly in plants.Journal of cell science · 2020Article
- Cooperativity in Proteasome Core Particle Maturation.iScience · 2020Article
- The Y. bercovieri Anbu crystal structure sheds light on the evolution of highly (pseudo)symmetric multimers.Journal of molecular biology · 2018Article
- Proteasome Structure and Assembly.Journal of molecular biology · 2017Review
- Evolution of the archaeal and mammalian information processing systems: towards an archaeal model for human disease.Cellular and molecular life sciences : CMLS · 2017Review
- Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach.Journal of visualized experiments : JoVE · 2016Article
- Precise assembly and regulation of 26S proteasome and correlation between proteasome dysfunction and neurodegenerative diseases.BMB reports · 2016Review
- Assembly of an Evolutionarily Conserved Alternative Proteasome Isoform in Human Cells.Cell reports · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Archaeal proteasomes share many features with their eukaryotic counterparts and serve as important models for assembly. Proteasomes are also found in certain bacterial lineages yet their assembly mechanism is thought to be fundamentally different. Here we investigate α-ring formation using recombinant proteasomes from the archaeon Methanococcus maripaludis. Through an engineered disulfide cross-linking strategy, we demonstrate that double α-rings are structurally analogous to half-proteasomes and can form independently of single α-rings. More importantly, via targeted mutagenesis, we show that single α-rings are not required for the efficient assembly of 20S proteasomes. Our data support updating the currently held "α-ring first" view of assembly, initially proposed in studies of archaeal proteasomes, and present a way to reconcile the seemingly separate bacterial assembly mechanism with the rest of the proteasome realm. We suggest that a common assembly network underpins the absolutely conserved architecture of proteasomes across all domains of life.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.