ArticleScientific reports2015
Gene looping facilitates TFIIH kinase-mediated termination of transcription.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 27 citations in OpenAlex.
- Protein-interaction network analysis reveals the role of Prp19 splicing factor in transcription of both intron-containing and intron-lacking genes.PLoS genetics · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- The general transcription factors (GTFs) of RNA polymerase II and their roles in plant development and stress responses.Critical reviews in biochemistry and molecular biology · 2024Review
- CSB-independent, XPC-dependent transcription-coupled repair inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Spatiotemporal coordination of transcription preinitiation complex assembly in live cells.Molecular cell · 2021Article
- Proximity to the Promoter and Terminator Regions Regulates the Transcription Enhancement Potential of an Intron.Frontiers in molecular biosciences · 2021Article
- RNA polymerase II phosphorylation and gene looping: new roles for the Rpb4/7 heterodimer in regulating gene expression.Current genetics · 2020Review
- Dissecting the Pol II transcription cycle and derailing cancer with CDK inhibitors.Nature chemical biology · 2020Review
- Chromosome conformation capture that detects novel cis- and trans-interactions in budding yeast.Methods (San Diego, Calif.) · 2020Article
- RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit.Nucleic acids research · 2019Article
- The essential and multifunctional TFIIH complex.Protein science : a publication of the Protein Society · 2018Review
- Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast.Scientific reports · 2017Article
- Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach.Journal of visualized experiments : JoVE · 2017Article
- Enhancement of Transcription by a Splicing-Competent Intron Is Dependent on Promoter Directionality.PLoS genetics · 2016Article
- The point of no return: The poly(A)-associated elongation checkpoint.RNA biology · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
TFIIH is a general transcription factor with kinase and helicase activities. The kinase activity resides in the Kin28 subunit of TFIIH. The role of Kin28 kinase in the early steps of transcription is well established. Here we report a novel role of Kin28 in the termination of transcription. We show that RNAPII reads through a termination signal upon kinase inhibition. Furthermore, the recruitment of termination factors towards the 3' end of a gene was compromised in the kinase mutant, thus confirming the termination defect. A concomitant decrease in crosslinking of termination factors near the 5' end of genes was also observed in the kinase-defective mutant. Simultaneous presence of termination factors towards both the ends of a gene is indicative of gene looping; while the loss of termination factor occupancy from the distal ends suggest the abolition of a looped gene conformation. Accordingly, CCC analysis revealed that the looped architecture of genes was severely compromised in the Kin28 kinase mutant. In a looping defective sua7-1 mutant, even the enzymatically active Kin28 kinase could not rescue the termination defect. These results strongly suggest a crucial role of Kin28 kinase-dependent gene looping in the termination of transcription in budding yeast.
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