ArticleBMC microbiology2015
Two nucleotide second messengers regulate the production of the Vibrio cholerae colonization factor GbpA.
Article in BMC microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Characterization of the dual regulation by a c-di-GMP riboswitch Bc1 with a long expression platform fromMicrobiology spectrum · 2024Article
- Bacterial Chitinases and Their Role in Human Infection.Infection and immunity · 2023Review
- DegS protease regulates the motility, chemotaxis, and colonization ofFrontiers in microbiology · 2023Article
- Development of Antibiofilm Therapeutics Strategies to Overcome Antimicrobial Drug Resistance.Microorganisms · 2022Review
- Article
- The cyanobacterial phytochrome 2 regulates the expression of motility-related genes through the second messenger cyclic di-GMP.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2020Article
- Cyclic di-GMP Increases Catalase Production and Hydrogen Peroxide Tolerance inApplied and environmental microbiology · 2019Article
- Transcription ofJournal of bacteriology · 2019Article
- Axenic Biofilm Formation and Aggregation byApplied and environmental microbiology · 2019Article
- Exploring the Links between Nucleotide Signaling and Quorum Sensing Pathways in Regulating Bacterial Virulence.ACS infectious diseases · 2018Review
- Article
- cAMP Receptor Protein Controls Vibrio cholerae Gene Expression in Response to Host Colonization.mBio · 2018Article
- c-di-GMP Regulates Various Phenotypes and Insecticidal Activity of Gram-PositiveFrontiers in microbiology · 2018Article
- A Nutrient-Regulated Cyclic Diguanylate Phosphodiesterase Controls Clostridium difficile Biofilm and Toxin Production during Stationary Phase.Infection and immunity · 2017Article
- Physiological and Molecular Understanding of Bacterial Polysaccharide Monooxygenases.Microbiology and molecular biology reviews : MMBR · 2017Review
- Cyclic diguanylate signaling in Gram-positive bacteria.FEMS microbiology reviews · 2016Review
- The RNA Domain Vc1 Regulates Downstream Gene Expression in Response to Cyclic Diguanylate in Vibrio cholerae.PloS one · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe nucleotide second messengers cAMP and c-di-GMP allow many bacteria, including the human intestinal pathogen Vibrio cholerae, to respond to environmental stimuli with appropriate physiological adaptations. In response to limitation of specific carbohydrates, cAMP and its receptor CRP control the transcription of genes important for nutrient acquisition and utilization; c-di-GMP controls the transition between motile and sessile lifestyles often, but not exclusively, through transcriptional mechanisms. In this study, we investigated the convergence of cAMP and c-di-GMP signaling pathways in regulating the expression of gbpA. GbpA is a colonization factor that participates in the attachment of V. cholerae to N-acetylglucosamine-containing surfaces in its native aquatic environment and the host intestinal tract.
resultsWe show that c-di-GMP inhibits gbpA activation in a fashion independent of the known transcription factors that directly sense c-di-GMP. Interestingly, inhibition of gbpA activation by c-di-GMP only occurs during growth on non-PTS dependent nutrient sources. Consistent with this result, we show that CRP binds to the gbpA promoter in a cAMP-dependent manner in vitro and drives transcription of gbpA in vivo. The interplay between cAMP and c-di-GMP does not broadly impact the CRP-cAMP regulon, but occurs more specifically at the gbpA promoter.
conclusionsThese findings suggest that c-di-GMP directly interferes with the interaction of CRP-cAMP and the gbpA promoter via an unidentified regulator. The use of two distinct second messenger signaling mechanisms to regulate gbpA transcription may allow V. cholerae to finely modulate GbpA production, and therefore colonization of aquatic and host surfaces, in response to discrete environmental stimuli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.