ArticleBMC microbiology2015
Contribution of the non-effector members of the HrpL regulon, iaaL and matE, to the virulence of Pseudomonas syringae pv. tomato DC3000 in tomato plants.
Article in BMC microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Vesicular and non-vesicular extracellular small RNAs direct gene silencing in a plant-interacting bacterium.Nature communications · 2025Article
- Genomic and mutational analysis of Pseudomonas syringae pv. tagetis EB037 pathogenicity on sunflower.BMC microbiology · 2025Article
- Multidrug and Toxic Compound Extrusion Transporters: Ubiquitous Multifaceted Proteins in Microbes, Plants, and Their Interactions.Microorganisms · 2024Review
- Article
- Allelic variation in the indoleacetic acid-lysine synthase gene of the bacterial pathogenFrontiers in plant science · 2023Article
- Identification of Indole-3-Acetic Acid-Regulated Genes inJournal of bacteriology · 2022Article
- A MATE Transporter is Involved in Pathogenicity and IAA Homeostasis in the Hyperplastic Plant PathogenMicroorganisms · 2020Article
- Re-programming of Pseudomonas syringae pv. actinidiae gene expression during early stages of infection of kiwifruit.BMC genomics · 2018Article
- Pseudomonas syringae: what it takes to be a pathogen.Nature reviews. Microbiology · 2018Review
- Genetic and Phenotypic Characterization of Indole-Producing Isolates ofFrontiers in microbiology · 2018Article
- Knots Untie: Molecular Determinants Involved in Knot Formation Induced byFrontiers in plant science · 2017Review
- Phytohormone pathways as targets of pathogens to facilitate infection.Plant molecular biology · 2016Review
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe phytohormone indole-3-acetic acid (IAA) is widely distributed among plant-associated bacteria. Certain strains of the Pseudomonas syringae complex can further metabolize IAA into a less biologically active amino acid conjugate, 3-indole-acetyl-ε-L-lysine, through the action of the iaaL gene. In P. syringae and Pseudomonas savastanoi strains, the iaaL gene is found in synteny with an upstream gene, here called matE, encoding a putative MATE family transporter. In P. syringae pv. tomato (Pto) DC3000, a pathogen of tomato and Arabidopsis plants, the HrpL sigma factor controls the expression of a suite of virulence-associated genes via binding to hrp box promoters, including that of the iaaL gene. However, the significance of HrpL activation of the iaaL gene in the virulence of Pto DC3000 is still unclear.
resultsA conserved hrp box motif is found upstream of the iaaL gene in the genomes of P. syringae strains. However, although the promoter region of matE is only conserved in genomospecies 3 of this bacterial group, we showed that this gene also belongs to the Pto DC3000 HrpL regulon. We also demonstrated that the iaaL gene is transcribed both independently and as part of an operon with matE in this pathogen. Deletion of either the iaaL or the matE gene resulted in reduced fitness and virulence of Pto DC3000 in tomato plants. In addition, we used multicolor fluorescence imaging to visualize the responses of tomato plants to wild-type Pto DC3000 and to its ΔmatE and ΔiaaL mutants. Activation of secondary metabolism prior to the development of visual symptoms was observed in tomato leaves after bacterial challenges with all strains. However, the observed changes were strongest in plants challenged by the wild-type strain, indicating lower activation of secondary metabolism in plants infected with the ΔmatE or ΔiaaL mutants.
conclusionsOur results provide new evidence for the roles of non-type III effector genes belonging to the Pto DC3000 HrpL regulon in virulence.
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