ArticleScientific reports2015
Investigation of Human Cancers for Retrovirus by Low-Stringency Target Enrichment and High-Throughput Sequencing.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Bovine leukemia virus (BLV) and risk of breast cancer; a systematic review and meta-analysis.Retrovirology · 2024Pooled it
- The viral origins of breast cancer.Infectious agents and cancer · 2024Review
- Mouse Mammary Tumour Virus (MMTV) in Human Breast Cancer-The Value of Bradford Hill Criteria.Viruses · 2022Review
- Identification of KIF4A and its effect on the progression of lung adenocarcinoma based on the bioinformatics analysis.Bioscience reports · 2021Article
- Enteric Virome and Carcinogenesis in the Gut.Digestive diseases and sciences · 2020Review
- Evidence for a causal role by human papillomaviruses in prostate cancer - a systematic review.Infectious agents and cancer · 2020Review
- High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach.The Journal of infectious diseases · 2019Article
- Application of a targeted-enrichment methodology for full-genome sequencing of Dengue 1-4, Chikungunya and Zika viruses directly from patient samples.PLoS neglected tropical diseases · 2019Article
- Metagenomic analysis of viruses in toilet waste from long distance flights-A new procedure for global infectious disease surveillance.PloS one · 2019Article
- Can Bovine Leukemia Virus Be Related to Human Breast Cancer? A Review of the Evidence.Journal of mammary gland biology and neoplasia · 2018Review
- Oncogenic Viruses and Breast Cancer: Mouse Mammary Tumor Virus (MMTV), Bovine Leukemia Virus (BLV), Human Papilloma Virus (HPV), and Epstein-Barr Virus (EBV).Frontiers in oncology · 2018Review
- Association of Mouse Mammary Tumor Virus With Human Breast Cancer: Histology, Immunohistochemistry and Polymerase Chain Reaction Analyses.Frontiers in oncology · 2018Article
- A universal primer-independent next-generation sequencing approach for investigations of norovirus outbreaks and novel variants.Scientific reports · 2017Article
- Multiple oncogenic viruses are present in human breast tissues before development of virus associated breast cancer.Infectious agents and cancer · 2017Article
- Mouse mammary tumor-like virus (MMTV) is present in human breast tissue before development of virally associated breast cancer.Infectious agents and cancer · 2017Article
- Substitutions of short heterologous DNA segments of intragenomic or extragenomic origins produce clustered genomic polymorphisms.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Identification of Viruses in Cases of Pediatric Acute Encephalitis and Encephalopathy Using Next-Generation Sequencing.Scientific reports · 2016Article
- Application of whole genome shotgun sequencing for detection and characterization of genetically modified organisms and derived products.Analytical and bioanalytical chemistry · 2016Review
- Application of targeted enrichment to next-generation sequencing of retroviruses integrated into the host human genome.Scientific reports · 2016Article
- Identification of Known and Novel Recurrent Viral Sequences in Data from Multiple Patients and Multiple Cancers.Viruses · 2016Article
Corrections and comments
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Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although nearly one fifth of all human cancers have an infectious aetiology, the causes for the majority of cancers remain unexplained. Despite the enormous data output from high-throughput shotgun sequencing, viral DNA in a clinical sample typically constitutes a proportion of host DNA that is too small to be detected. Sequence variation among virus genomes complicates application of sequence-specific, and highly sensitive, PCR methods. Therefore, we aimed to develop and characterize a method that permits sensitive detection of sequences despite considerable variation. We demonstrate that our low-stringency in-solution hybridization method enables detection of <100 viral copies. Furthermore, distantly related proviral sequences may be enriched by orders of magnitude, enabling discovery of hitherto unknown viral sequences by high-throughput sequencing. The sensitivity was sufficient to detect retroviral sequences in clinical samples. We used this method to conduct an investigation for novel retrovirus in samples from three cancer types. In accordance with recent studies our investigation revealed no retroviral infections in human B-cell lymphoma cells, cutaneous T-cell lymphoma or colorectal cancer biopsies. Nonetheless, our generally applicable method makes sensitive detection possible and permits sequencing of distantly related sequences from complex material.
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