ArticleThe Journal of biological chemistry2015
Regulation of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase: product inhibition, cooperativity, and magnesium activation.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 79 citations in OpenAlex.
- Critical Leaf Magnesium Thresholds and the Impact of Magnesium on Plant Growth and Photo-Oxidative Defense: A Systematic Review and Meta-Analysis From 70 Years of Research.Frontiers in plant science · 2019Pooled it
- Overexpression of Rubisco Activase Improves Photosynthesis and Plant Growth in Rice Under Fluctuating Light Under Future High COPlant, cell & environment · 2026Article
- Magnesium-mediated stress adaptation in plants: from physio-biochemical insights to climate-resilient agriculture.Frontiers in plant science · 2026Review
- Two cowpea Rubisco activase isoforms for crop thermotolerance.The New phytologist · 2025Article
- Ribulose-1,5-bisphosphate carboxylase/oxygenase activase isoforms from diverse species show differences in oligomeric structure, thermal stability, and activity.European biophysics journal : EBJ · 2025Article
- The heat is on: scaling improvements in photosynthetic thermal tolerance from the leaf to canopy to predict crop yields in a changing climate.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- C4 grasses employ distinct strategies to acclimate rubisco activase to heat stress.Bioscience reports · 2024Article
- Perspectives on improving photosynthesis to increase crop yield.The Plant cell · 2024Review
- Molecular mechanism of Rubisco activase: Dynamic assembly and Rubisco remodeling.Frontiers in molecular biosciences · 2023Review
- Membrane destabilization and pore formation induced by the Synechocystis IM30 protein.Biophysical journal · 2022Article
- Physiological Essence of Magnesium in Plants and Its Widespread Deficiency in the Farming System of China.Frontiers in plant science · 2022Review
- Magnesium Foliar Supplementation Increases Grain Yield of Soybean and Maize by Improving Photosynthetic Carbon Metabolism and Antioxidant Metabolism.Plants (Basel, Switzerland) · 2021Article
- Magnesium Application Promotes Rubisco Activation and Contributes to High-Temperature Stress Alleviation in Wheat During the Grain Filling.Frontiers in plant science · 2021Article
- Structural asymmetry does not indicate hemiphosphorylation in the bacterial histidine kinase CpxA.The Journal of biological chemistry · 2020Article
- A single point mutation in the C-terminal extension of wheat Rubisco activase dramatically reduces ADP inhibition via enhanced ATP binding affinity.The Journal of biological chemistry · 2019Article
- Regulation of the leaf proteome by inoculation of Populus × canescens with two Paxillus involutus isolates differing in root colonization rates.Mycorrhiza · 2019Article
- Assembly-disassembly is coupled to the ATPase cycle of tobacco Rubisco activase.The Journal of biological chemistry · 2018Article
- In Vitro Characterization of Thermostable CAM Rubisco Activase Reveals a Rubisco Interacting Surface Loop.Plant physiology · 2017Article
- Rubisco Activases: AAA+ Chaperones Adapted to Enzyme Repair.Frontiers in molecular biosciences · 2017Review
- The Diverse AAA+ Machines that Repair Inhibited Rubisco Active Sites.Frontiers in molecular biosciences · 2017Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many photosynthetic organisms, tight-binding Rubisco inhibitors are released by the motor protein Rubisco activase (Rca). In higher plants, Rca plays a pivotal role in regulating CO2 fixation. Here, the ATPase activity of 0.005 mm tobacco Rca was monitored under steady-state conditions, and global curve fitting was utilized to extract kinetic constants. The kcat was best fit by 22.3 ± 4.9 min(-1), the Km for ATP by 0.104 ± 0.024 mm, and the Ki for ADP by 0.037 ± 0.007 mm. Without ADP, the Hill coefficient for ATP hydrolysis was extracted to be 1.0 ± 0.1, indicating noncooperative behavior of homo-oligomeric Rca assemblies. However, the addition of ADP was shown to introduce positive cooperativity between two or more subunits (Hill coefficient 1.9 ± 0.2), allowing for regulation via the prevailing ATP/ADP ratio. ADP-mediated activation was not observed, although larger amounts led to competitive product inhibition of hydrolytic activity. The catalytic efficiency increased 8.4-fold upon cooperative binding of a second magnesium ion (Hill coefficient 2.5 ± 0.5), suggesting at least three conformational states (ATP-bound, ADP-bound, and empty) within assemblies containing an average of about six subunits. The addition of excess Rubisco (24:1, L8S8/Rca6) and crowding agents did not modify catalytic rates. However, high magnesium provided for thermal Rca stabilization. We propose that magnesium mediates the formation of closed hexameric toroids capable of high turnover rates and amenable to allosteric regulation. We suggest that in vivo, the Rca hydrolytic activity is tuned by fluctuating [Mg(2+)] in response to changes in available light.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.