Evidence map›Paper›PMID 26283270›Full record

ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2015

Histone acetylation regulates prostate ductal morphogenesis through a bone morphogenetic protein-dependent mechanism.

Kimberly P Keil, Helene M Altmann, Lisa L Abler, Laura L Hernandez, Chad M Vezina

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Trial
  2. Review
  3. Regulation of bone morphogenetic protein 4 on epithelial tissue.Journal of cell communication and signaling · 2020
    Review
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Kimberly P KeilDepartment of Comparative Biosciences, University of Wisconsin-Madison, Madison, Wisconsin.
Helene M AltmannDepartment of Comparative Biosciences, University of Wisconsin-Madison, Madison, Wisconsin.
Lisa L AblerDepartment of Comparative Biosciences, University of Wisconsin-Madison, Madison, Wisconsin.
Laura L HernandezDepartment of Dairy Science, University of Wisconsin-Madison, Madison, Wisconsin.
Chad M VezinaDepartment of Comparative Biosciences, University of Wisconsin-Madison, Madison, Wisconsin.
University of Wisconsin–Madison · USUniversity of Wisconsin System · US

Funding

Role of DNA methylation in prostate glandular development and urinary functionR01DK099328 · NIDDK · UNIVERSITY OF WISCONSIN-MADISON · PI VEZINA, CHAD M. · 2014 to 2018
$1.6M
NIDDK NIH HHS R01 DK099328NIDDK NIH HHS R01DK099328
6 · The paper itself

Abstract

backgroundEpigenetic factors influence stem cell function and other developmental events but their role in prostate morphogenesis is not completely known. We tested the hypothesis that histone deacetylase (HDAC) activity is required for prostate morphogenesis.

resultsWe identified the presence of class I nuclear HDACs in the mouse urogenital sinus (UGS) during prostate development and found that Hdac 2 mRNA abundance diminishes as development proceeds which is especially evident in prostatic epithelium. Blockade of HDACs with the inhibitor trichostatin A (TSA) decreased the number of prostatic buds formed in UGS explant cultures but not the number of buds undergoing branching morphogenesis. In the latter, TSA promoted an extensive branching phenotype that was reversed by exogenous NOGGIN protein, which functions as a bone morphogenetic protein (BMP) inhibitor. TSA also increased Bmp2 promoter H3K27ac abundance, Bmp2 and Bmp4 mRNA abundance, and the percentage of epithelial cells marked by BMP-responsive phosphorylated SMAD1/5/8 protein. TSA exposed UGS explants grafted under the kidney capsule of untreated host mice for continued development achieved a smaller size without an obvious difference in glandular histology compared with control treated grafts.

conclusionsThese results are consistent with an active role for HDACs in shaping prostate morphogenesis by regulating Bmp abundance.

Indexed as

AcetylationAnimalsBone Morphogenetic Protein 2Bone Morphogenetic Protein 4Bone Morphogenetic ProteinsChromatinChromatin ImmunoprecipitationEpigenesis, GeneticGene Expression Regulation, DevelopmentalHistone Deacetylase 2HistonesHumansHydroxamic AcidsImmunohistochemistryIn Situ HybridizationMaleBMP2 protein, humanBmp2 protein, mouseBmp4 protein, mouseBone Morphogenetic Protein 2Bone Morphogenetic Protein 4Bone Morphogenetic ProteinsChromatinHdac2 protein, mouseHistone Deacetylase 2HistonesHydroxamic AcidsRecombinant ProteinsRNA, Messengertrichostatin Achromatintrichostatin Aurogenital sinus

Identifiers

PMID26283270
PMCPMC4619142
OpenAlexW2168614904

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.