Evidence map›Paper›PMID 26283164›Full record

ArticleActa biomaterialia2015

Porous nanofibrous poly(L-lactic acid) scaffolds supporting cardiovascular progenitor cells for cardiac tissue engineering.

Qihai Liu, Shuo Tian, Chao Zhao, Xin Chen, Ienglam Lei, Zhong Wang, Peter X Ma

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Article
  7. Review
  8. Progress in preclinical research on induced pluripotent stem cell therapy for acute myocardial infarction.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2024
    Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Progress in Bioengineering Strategies for Heart Regenerative Medicine.International journal of molecular sciences · 2022
    Review
  14. Review
  15. Review
  16. Review
  17. Review
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Qihai LiuDepartment of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, MI 48109, USA.
Shuo TianDepartment of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, MI 48109, USA.
Chao ZhaoDepartment of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, MI 48109, USA.
Xin ChenDepartment of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, MI 48109, USA.
Ienglam LeiDepartment of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, MI 48109, USA.
Zhong WangDepartment of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: zhongw@umich.edu.
Peter X MaDepartment of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, The University of Michigan, Ann Arbor, MI 48109, USA; Macromolecular Science and Engineering Center, The University of Michigan, Ann Arbor, MI 48109, USA; Department of Materials Science and Engineering, The University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: mapx@umich.edu.

Funding

Regenerating Blood Vessels Using iPS CellsR01HL114038 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MA, PETER X · 2013 to 2016
$2.1M
Epigenetic control of multipotent cardiac progenitor cell differentiationR01HL109054 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WANG, ZHONG · 2012 to 2016
$1.9M
NHLBI NIH HHS HL109054NHLBI NIH HHS HL114038NHLBI NIH HHS R01 HL109054NHLBI NIH HHS R01 HL114038
6 · The paper itself

Abstract

Myocardial infarction (MI) is the irreversible necrosis of heart with approximately 1.5 million cases every year in the United States. Tissue engineering offers a promising strategy for cardiac repair after MI. However, the optimal cell source for heart tissue regeneration and the ideal scaffolds to support cell survival, differentiation, and integration, remain to be developed. To address these issues, we developed the technology to induce cardiovascular progenitor cells (CPCs) derived from mouse embryonic stem cells (ESCs) towards desired cardiomyocytes as well as smooth muscle cells and endothelial cells. We fabricated extracellular matrix (ECM)-mimicking nanofibrous poly(l-lactic acid) (PLLA) scaffolds with porous structure of high interconnection for cardiac tissue formation. The CPCs were seeded into the scaffolds to engineer cardiac constructs in vitro. Fluorescence staining and RT-PCR assay showed that the scaffolds facilitated cell attachment, extension, and differentiation. Subcutaneous implantation of the cell/scaffold constructs in a nude mouse model showed that the scaffolds favorably supported survival of the grafted cells and their commitment to the three desired lineages in vivo. Thus, our study suggested that the porous nanofibrous PLLA scaffolds support cardiac tissue formation from CPCs. The integration of CPCs with the nanofibrous PLLA scaffolds represents a promising tissue engineering strategy for cardiac repair. STATEMENT OF SIGNIFICANCE: Myocardial infarction is the irreversible necrosis of heart with approximately 1.5 million cases every year in the United States. Tissue engineering offers a promising strategy for cardiac repair after MI. However, the optimal cell source for heart tissue regeneration and the ideal scaffolds to support cell survival, differentiation, and integration, remain to be developed. To address these issues, we developed porous nanofibrous PLLA scaffolds that mimic natural extracellular matrix to support cardiac tissue formation from CPCs. The integration of CPCs with the nanofibrous PLLA scaffolds represents a promising tissue engineering strategy for cardiac repair.

Indexed as

Tissue ScaffoldsAnimalsCells, CulturedEquipment DesignEquipment Failure AnalysisGuided Tissue RegenerationHeartLactic AcidMaterials TestingMiceMice, NudeMyocardiumNanofibersNanoporesPolyestersPolymersLactic AcidPolyesterspoly(lactide)PolymersCardiac differentiationCardiac tissue engineeringCardiovascular progenitor cellEmbryonic stem cellExtracellular matrixPorous nanofibrous scaffold

Identifiers

PMID26283164
PMCPMC4584192

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.