ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2015
Development and evaluation of MALDI-TOF MS-based serotyping for Streptococcus pneumoniae.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A systematic review of machine learning on clinical MALDI-TOF MS.Briefings in bioinformatics · 2026Pooled it
- Promising potential of machine learning-assisted MALDI-TOF MS as an effective detector forApplied and environmental microbiology · 2023Article
- ExploringJournal of mass spectrometry and advances in the clinical lab · 2023Article
- Use of MALDI-TOF MS to Discriminate between Aflatoxin B1-Producing and Non-Producing Strains ofMolecules (Basel, Switzerland) · 2022Article
- Burden of illness associated with pneumococcal infections in Japan - a targeted literature review.Journal of market access & health policy · 2022Review
- Rapid Identification ofFrontiers in microbiology · 2022Article
- Investigation of MALDI-TOF Mass Spectrometry for Assessing the Molecular Diversity ofDiagnostics (Basel, Switzerland) · 2021Article
- MALDI-TOF mass spectrometry for sub-typing of Streptococcus pneumoniae.BMC microbiology · 2020Article
- Rapid Classification of Multilocus Sequence Subtype for Group BFrontiers in cellular and infection microbiology · 2020Article
- Evaluation of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry and ClinPro Tools as a Rapid Tool for TypingOpen forum infectious diseases · 2019Article
- Article
- Discrimination of contagious and environmental strains of Streptococcus uberis in dairy herds by means of mass spectrometry and machine-learning.Scientific reports · 2018Article
- Rapid and robust MALDI-TOF MS techniques for microbial identification: a brief overview of their diverse applications.Journal of microbiology (Seoul, Korea) · 2018Review
- Potential of MALDI-TOF MS as an alternative approach for capsular typing Streptococcus pneumoniae isolates.Scientific reports · 2017Article
- Elucidating constraints for differentiation of major human Klebsiella pneumoniae clones using MALDI-TOF MS.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
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Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Surveillance of Streptococcus pneumoniae serotypes is important for the successful implementation of vaccination strategies to prevent the spread of invasive pneumococcal diseases. The standard method of serotyping of pneumococcal isolates is the phenotypic Neufeld test, which is cost- and labor-intensive. Recently, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been implemented as a rapid, simple and inexpensive method for identifying species. We evaluated the performance of MALDI-TOF MS for serotyping ten major serotypes of S. pneumoniae in Japan (serotypes 3, 6B, 15A, 15C, 19A, 19 F, 23A, 24 F, 35B and 38) using the Biotyper and ClinProTools. After optimizing the settings, we validated their serotyping performance for serotypes 3, 15A and 19A using a separate set of isolates that were not used in the creation of the classification algorithms. A total of 574 isolates of S. pneumoniae collected from Japanese nationwide surveillance studies were included. Of these, 407 isolates belonged to the ten major serotypes. Biotyper and ClinProTools correctly identified 77.9 % and 84.0 %, respectively, of the ten major serotype isolates. The validation analysis included a total of 113 isolates of the serotypes 3, 15A and 19A isolates. Biotyper and ClinProTools correctly identified 85.0 % and 69.9 % of the validation cohort isolates, respectively. MALDI-TOF MS has the potential to discriminate the ten major S. pneumoniae serotypes prevalent in Japan.
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