Evidence map›Paper›PMID 26282538›Full record

ArticleStem cell research & therapy2015

Mesenchymal stem cells derived from human induced pluripotent stem cells retain adequate osteogenicity and chondrogenicity but less adipogenicity.

Ran Kang, Yan Zhou, Shuang Tan, Guangqian Zhou, Lars Aagaard, Lin Xie, Cody Bünger, Lars Bolund, Yonglun Luo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
78citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

78 citing papers in PubMed, 1 synthesis or guideline pooled it.

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  13. 3D Culture of MSCs for Clinical Application.Bioengineering (Basel, Switzerland) · 2024
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18 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ran KangOrthopedic Research Lab, Aarhus University, 8000, Aarhus C, Denmark. KANG.RAN@CLIN.AU.DK.
Yan ZhouDepartment of Biomedicine, the Health Faculty, Aarhus University, 8000, Aarhus C, Denmark. YAZH@BIOMED.AU.DK.
Shuang TanDepartment of Biomedicine, the Health Faculty, Aarhus University, 8000, Aarhus C, Denmark. SHUANG.TAN@HUM-GEN.AU.DK.
Guangqian ZhouShenzhen Key Laboratory for Anti-aging and Regenerative Medicine, Health Science Center, Shenzhen University, 518060, Shenzhen, China. GQZHOU@SZU.EDU.CN.
Lars AagaardDepartment of Biomedicine, the Health Faculty, Aarhus University, 8000, Aarhus C, Denmark. AAGAARD@BIOMED.AU.DK.
Lin XieJiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, 210028, China. XIELIN117@126.COM.
Cody BüngerOrthopedic Research Lab, Aarhus University, 8000, Aarhus C, Denmark. CODYBUNG@RM.DK.
Lars BolundDepartment of Biomedicine, the Health Faculty, Aarhus University, 8000, Aarhus C, Denmark. BOLUND@BIOMED.AU.DK.
Yonglun LuoDepartment of Biomedicine, the Health Faculty, Aarhus University, 8000, Aarhus C, Denmark. ALUN@BIOMED.AU.DK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionPreviously, we established a simple method for deriving mesenchymal stem cells (MSCs) from human induced pluripotent stem cells (iPSC-MSCs). These iPSC-MSCs were capable of forming osteogenic structures in scaffolds and nanofibers. The objective of this study is to systematically characterize the mesenchymal characteristics of the iPSC-MSCs by comparing them to bone marrow-derived MSCs (BM-MSCs).

methodsTwo iPSC-MSC lines (named as mRNA-iPSC-MSC-YL001 and lenti-iPSC-MSC-A001) and one BM-MSC line were used for the study. Cell proliferation, presence of mesenchymal surface markers, tri-lineage differentiation capability (osteogenesis, chondrogenesis, adipogenesis), and expression of "stemness" genes were analyzed in these MSC lines.

resultsThe iPSC-MSCs were similar to BM-MSCs in terms of cell morphology (fibroblast-like) and surface antigen profile: CD29+, CD44+, CD73+, CD90+, CD105+, CD11b-, CD14-, CD31-, CD34-, CD45- and HLA-DR-. A faster proliferative capability was seen in both iPSC-MSCs lines compared to the BM-MSCs. The iPSC-MSCs showed adequate capacity of osteogenesis and chondrogenesis compared to the BM-MSCs, while less adipogenic potential was found in the iPSC-MSCs. The iPSC-MSCs and the tri-lineage differentiated cells (osteoblasts, chondrocytes, adipocytes) all lack expression of "stemness" genes: OCT4, SOX2, GDF3, CRIPTO, UTF1, DPPA4, DNMT3B, LIN28a, and SAL4.

conclusionsThe MSCs derived from human iPSCs with our method have advanced proliferation capability and adequate osteogenic and chondrogenic properties compared to BM-MSCs. However, the iPSC-MSCs were less efficient in their adipogenicity, suggesting that further modifications should be applied to our method to derive iPSC-MSCs more closely resembling the naïve BM-MSCs if necessary.

Indexed as

AdipogenesisCell DifferentiationCell LineCells, CulturedChondrogenesisFlow CytometryHEK293 CellsHumansInduced Pluripotent Stem CellsLentivirusMesenchymal Stem CellsOsteogenesis

Identifiers

PMID26282538
PMCPMC4539932

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.