ArticleNature communications2015
Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed.
- Developing Optogenetic Approaches to Study CaCold Spring Harbor perspectives in biology · 2026Review
- Molecular jackhammers induce intracellular calcium release and skeletal muscle contraction by vibronic-driven action.Chemical science · 2026Article
- Interrogating Physiological Functions with Light and Chemicals.Annual review of physiology · 2026Review
- Optogenetic control of T cells for immunomodulation.Essays in biochemistry · 2025Review
- Optogenetic engineering for ion channel modulation.Current opinion in chemical biology · 2025Review
- Spontaneous Calcium Bursts Organize the Apical Actin Cytoskeleton of Multiciliated Cells.International journal of molecular sciences · 2025Article
- STIM Proteins: The Gas and Brake of Calcium Entry in Neurons.Neuroscience bulletin · 2025Review
- Advances in Metal Halide Perovskite Memristors: A Review from a Co-Design Perspective.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- ORAI CaBiomolecules · 2024Review
- Synthetic Biology Meets CaCells · 2024Review
- Development of an optogenetics tool, Opto-RANK, for control of osteoclast differentiation using blue light.Scientific reports · 2024Article
- Programmable synthetic receptors: the next-generation of cell and gene therapies.Signal transduction and targeted therapy · 2024Review
- Cardiac optogenetics: shining light on signaling pathways.Pflugers Archiv : European journal of physiology · 2023Review
- AAV-compatible optogenetic tools for activating endogenous calcium channels in vivo.Molecular brain · 2023Article
- RCN2 promotes Nasopharyngeal carcinoma progression by curbing Calcium flow and Mitochondrial apoptosis.Cellular oncology (Dordrecht, Netherlands) · 2023Article
- Proteolytically Activated CRAC Effectors through Designed Intramolecular Inhibition.ACS synthetic biology · 2022Article
- Optophysiology: Illuminating cell physiology with optogenetics.Physiological reviews · 2022Review
- Photopharmacological modulation of native CRAC channels using azoboronate photoswitches.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Arbitrary CaFrontiers in pharmacology · 2022Article
- Deciphering Molecular Mechanisms and Intervening in Physiological and Pathophysiological Processes of CaCells · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ca(2+) signals are highly regulated in a spatiotemporal manner in numerous cellular physiological events. Here we report a genetically engineered blue light-activated Ca(2+) channel switch (BACCS), as an optogenetic tool for generating Ca(2+) signals. BACCS opens Ca(2+)-selective ORAI ion channels in response to light. A BACCS variant, dmBACCS2, combined with Drosophila Orai, elevates the Ca(2+) concentration more rapidly, such that Ca(2+) elevation in mammalian cells is observed within 1 s on light exposure. Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression. In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses. Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca(2+). Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca(2+) signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.