ArticlePlant physiology2015
Direct Recording of Trans-Plasma Membrane Electron Currents Mediated by a Member of the Cytochrome b561 Family of Soybean.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- The Origin of Dielectric Permittivity in Plants.International journal of molecular sciences · 2026Review
- Electron currents mediated by tonoplast cytochromes b561.European biophysics journal : EBJ · 2026Review
- Plasma membrane CYBDOM proteins catalyse apoplastic AsA regeneration and interact with RbohD to activate autophagy and drought tolerance in plants.Nature plants · 2025Article
- Tonoplast-targeted bacterial transporter MerC enhances cadmium tolerance in Arabidopsis via vacuolar sequestration and cytoplasmic protection.Plant cell reports · 2025Article
- The Vacuolar Inositol Transporter BvINT1;1 Contributes to Raffinose Biosynthesis and Reactive Oxygen Species Scavenging During Cold Stress in Sugar Beet.Plant, cell & environment · 2025Article
- Ferric reduction by a CYBDOM protein counteracts increased iron availability in root meristems induced by phosphorus deficiency.Nature communications · 2024Article
- A CYBDOM protein impacts iron homeostasis and primary root growth under phosphate deficiency in Arabidopsis.Nature communications · 2024Article
- Natural variation in CRABS CLAW contributes to fruit length divergence in cucumber.The Plant cell · 2023Article
- Electrophysiology and fluorescence to investigate cation channels and transporters in isolated plant vacuoles.Stress biology · 2022Review
- Review
- Membrane voltage as a dynamic platform for spatiotemporal signaling, physiological, and developmental regulation.Plant physiology · 2021Article
- Biomolecular Basis of Cellular Consciousness via Subcellular Nanobrains.International journal of molecular sciences · 2021Review
- Role of Transportome in the Gills of Chinese Mitten Crabs in Response to Salinity Change: A Meta-Analysis of RNA-Seq Datasets.Biology · 2021Article
- Loss of a highly conserved sterile alpha motif domain gene (Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trans-plasma membrane electron transfer is achieved by b-type cytochromes of different families, and plays a fundamental role in diverse cellular processes involving two interacting redox couples that are physically separated by a phospholipid bilayer, such as iron uptake and redox signaling. Despite their importance, no direct recordings of trans-plasma membrane electron currents have been described in plants. In this work, we provide robust electrophysiological evidence of trans-plasma membrane electron flow mediated by a soybean (Glycine max) cytochrome b561 associated with a dopamine β-monooxygenase redox domain (CYBDOM), which localizes to the plasma membrane in transgenic Arabidopsis (Arabidopsis thaliana) plants and CYBDOM complementary RNA-injected Xenopus laevis oocytes. In oocytes, two-electrode voltage clamp experiments showed that CYBDOM-mediated currents were activated by extracellular electron acceptors in a concentration- and type-specific manner. Current amplitudes were voltage dependent, strongly potentiated in oocytes preinjected with ascorbate (the canonical electron donor for cytochrome b561), and abolished by mutating a highly conserved His residue (H292L) predicted to coordinate the cytoplasmic heme b group. We believe that this unique approach opens new perspectives in plant transmembrane electron transport and beyond.
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