Evidence map›Paper›PMID 26280331›Full record

ArticleNature methods2015

ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors.

Christian Schmidl, André F Rendeiro, Nathan C Sheffield, Christoph Bock

Abstract read
In one paragraph

Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 310 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
310citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

310 citing papers in PubMed, 1 synthesis or guideline pooled it.

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250 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Christian Schmidl *CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
André F Rendeiro *CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
Nathan C SheffieldCeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
Christoph BockCeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.

Funding

Austrian Science Fund FWF I 1626
6 · The paper itself

Abstract

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase ('tagmentation'). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.

Indexed as

Chromatin ImmunoprecipitationGenome, HumanHistonesHumansK562 CellsTranscription FactorsHistonesTranscription Factors

Identifiers

PMID26280331
PMCPMC4589892

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.