Evidence map›Paper›PMID 26280226›Full record

ArticleNature materials2015

Phage-mediated counting by the naked eye of miRNA molecules at attomolar concentrations in a Petri dish.

Xin Zhou, Peng Cao, Ye Zhu, Wuguang Lu, Ning Gu, Chuanbin Mao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature materials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Review
  6. Article
  7. T7 Phage as an Emerging Nanobiomaterial with Genetically Tunable Target Specificity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022
    Review
  8. Review
  9. Review
  10. Review
  11. Article
  12. Phage engineering and the evolutionary arms race.Current opinion in biotechnology · 2021
    Review
  13. Article
  14. Enhancement of Photodynamic Cancer Therapy by Physical and Chemical Factors.Angewandte Chemie (International ed. in English) · 2019
    Review
  15. Article
  16. Review
  17. Review
  18. Article
  19. Phage-Enabled Nanomedicine: From Probes to Therapeutics in Precision Medicine.Angewandte Chemie (International ed. in English) · 2017
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xin ZhouState Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Peng CaoLaboratory of Cellular and Molecular Biology, Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing 210028, China.
Ye ZhuDepartment of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019-5300, USA.
Wuguang LuLaboratory of Cellular and Molecular Biology, Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing 210028, China.
Ning GuState Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Chuanbin MaoDepartment of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019-5300, USA.

Funding

Tubulin-Binding Upconversion Nanoparticles for Breast-Cancer Imaging and TherapyR21EB015190 · NIBIB · UNIVERSITY OF OKLAHOMA · PI MAO, CHUANBIN · 2012 to 2013
$398k
Selective tumor inhibition by tumor-homing angiogenesis-suppressing nanofibersR21CA200504 · NCI · UNIVERSITY OF OKLAHOMA · PI MAO, CHUANBIN · 2015 to 2016
$361k
NCI NIH HHS CA200504NCI NIH HHS R21 CA200504NIBIB NIH HHS EB015190NIBIB NIH HHS R21 EB015190
6 · The paper itself

Abstract

The ability to count biomolecules such as cancer-biomarker miRNAs with the naked eye is seemingly impossible in molecular diagnostics. Here, we show an ultrasensitive naked-eye-counting strategy for quantifying miRNAs by employing T7 phage-a bacteria-specific virus nanoparticle-as a surrogate. The phage is genetically engineered to become fluorescent and capable of binding a miRNA-capturing gold nanoparticle (GNP) in a one-to-one manner. Target miRNAs crosslink the resultant phage-GNP couple and miRNA-capturing magnetic microparticles, forming a sandwich complex containing equimolar phage and miRNA. The phage is then released from the complex and developed into one macroscopic fluorescent plaque in a Petri dish by plating it in a host bacterial medium. Counting the plaques by the naked eye enables the quantification of miRNAs with detection limits of ∼3 and ∼5 aM for single-target and two-target miRNAs, respectively. This approach offers ultrasensitive and convenient quantification of disease biomarkers by the naked eye.

Indexed as

BacteriaBacteriophagesBacteriophage T7BiomarkersBiomarkers, TumorDNAFluorescent DyesGenome, BacterialGoldHumansMetal NanoparticlesMicroRNAsMicroscopy, Electron, TransmissionNanotechnologyNeoplasmsOligonucleotidesBiomarkersBiomarkers, TumorDNAFluorescent DyesGoldMicroRNAsOligonucleotides

Identifiers

PMID26280226
PMCPMC4924527

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.