ArticleEpigenetics & chromatin2015
Role of Ccr4-Not complex in heterochromatin formation at meiotic genes and subtelomeres in fission yeast.
Article in Epigenetics & chromatin, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 47 citations in OpenAlex.
- Spliceosomal proteins direct RNA methylation to modulate gene expression and silence retrotransposons.Nature communications · 2026Article
- The CCR4-NOT complex: a multifaceted sensor of molecular signals instructing eukaryotic mRNA translation and stability.Nucleic acids research · 2025Review
- Fission yeast Caprin protein is required for efficient heterochromatin establishment.PLoS genetics · 2025Article
- Article
- Review
- A dual, catalytic role for the fission yeast Ccr4-Not complex in gene silencing and heterochromatin spreading.Genetics · 2023Article
- Regulation of eukaryotic mRNA deadenylation and degradation by the Ccr4-Not complex.Frontiers in cell and developmental biology · 2023Review
- Proteomic analysis defines the interactome of telomerase in the protozoan parasite,Frontiers in cell and developmental biology · 2023Article
- Ccr4-Not complex reduces transcription efficiency in heterochromatin.Nucleic acids research · 2022Article
- TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.Nature communications · 2022Article
- R-loops and regulatory changes in chronologically ageing fission yeast cells drive non-random patterns of genome rearrangements.PLoS genetics · 2021Article
- Long Non-Coding RNAs in the Control of Gametogenesis: Lessons from Fission Yeast.Non-coding RNA · 2021Review
- A scaffold lncRNA shapes the mitosis to meiosis switch.Nature communications · 2021Article
- Review
- Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast.Nucleic acids research · 2020Article
- Formation of S. pombe Erh1 homodimer mediates gametogenic gene silencing and meiosis progression.Scientific reports · 2020Article
- Nuclear Ccr4-Not mediates the degradation of telomeric and transposon transcripts at chromatin in the Drosophila germline.Nucleic acids research · 2020Article
- meiRNA, A Polyvalent Player in Fission Yeast Meiosis.Non-coding RNA · 2019Review
- A conserved dimer interface connects ERH and YTH family proteins to promote gene silencing.Nature communications · 2019Article
- Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundHeterochromatin is essential for chromosome segregation, gene silencing and genome integrity. The fission yeast Schizosaccharomyces pombe contains heterochromatin at centromeres, subtelomeres, and mating type genes, as well as at small islands of meiotic genes dispersed across the genome. This heterochromatin is generated by partially redundant mechanisms, including the production of small interfering RNAs (siRNAs) that are incorporated into the RITS protein complex (RNAi-Induced Transcriptional Silencing). The assembly of heterochromatin islands requires the function of the RNA-binding protein Mmi1, which recruits RITS to its mRNA targets and to heterochromatin islands. In addition, Mmi1 directs its targets to an exosome-dependent RNA elimination pathway.
resultsCcr4-Not is a conserved multiprotein complex that regulates gene expression at multiple levels, including RNA degradation and translation. We show here that Ccr4-Not is recruited by Mmi1 to its RNA targets. Surprisingly, Ccr4 and Caf1 (the mRNA deadenylase catalytic subunits of the Ccr4-Not complex) are not necessary for the degradation or translation of Mmi1 RNA targets, but are essential for heterochromatin integrity at Mmi1-dependent islands and, independently of Mmi1, at subtelomeric regions. Both roles require the deadenylase activity of Ccr4 and the Mot2/Not4 protein, a ubiquitin ligase that is also part of the complex. Genetic evidence shows that Ccr4-mediated silencing is essential for normal cell growth, indicating that this novel regulation is physiologically relevant. Moreover, Ccr4 interacts with components of the RITS complex in a Mmi1-independent manner.
conclusionsTaken together, our results demonstrate that the Ccr4-Not complex is required for heterochromatin integrity in both Mmi1-dependent and Mmi1-independent pathways.
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