ArticleBiotechnology for biofuels2015
Cellobionic acid utilization: from Neurospora crassa to Saccharomyces cerevisiae.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- Transcription factor MtCLR-2 regulates cellulase production via direct modulation of Mtegl2 and Mtbgl1 expression in Myceliophthora thermophila.Microbial cell factories · 2026Article
- Correlating sugar transporter expression and activities to identify transporters for an orphan sugar substrate.Applied microbiology and biotechnology · 2024Article
- Role of cellulose response transporter-like protein CRT2 in cellulase induction in Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2023Article
- Bioproduction and applications of aldobionic acids with a focus on maltobionic and cellobionic acid.Bioprocess and biosystems engineering · 2023Review
- Effect of itaconic acid production on Neurospora crassa in consolidated bioprocessing of cellulose.Microbial cell factories · 2023Article
- Functional characterization of a highly specific L-arabinose transporter from Trichoderma reesei.Microbial cell factories · 2021Article
- Datamining and functional environmental genomics reassess the phylogenetics and functional diversity of fungal monosaccharide transporters.Applied microbiology and biotechnology · 2021Article
- The regulatory and transcriptional landscape associated with carbon utilization in a filamentous fungus.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Homoethanol Production from Glycerol and Gluconate Using RecombinantApplied and environmental microbiology · 2019Article
- Efficient production of sugar-derived aldonic acids byRSC advances · 2018Article
- A fungal transcription factor essential for starch degradation affects integration of carbon and nitrogen metabolism.PLoS genetics · 2017Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEconomical production of fuels and chemicals from plant biomass requires the efficient use of sugars derived from the plant cell wall. Neurospora crassa, a model lignocellulosic degrading fungus, is capable of breaking down the complex structure of the plant cell wall. In addition to cellulases and hemicellulases, N. crassa secretes lytic polysaccharide monooxygenases (LPMOs), which cleave cellulose by generating oxidized sugars-particularly aldonic acids. However, the strategies N. crassa employs to utilize these sugars are unknown.
resultsWe identified an aldonic acid utilization pathway in N. crassa, comprised of an extracellular hydrolase (NCU08755), cellobionic acid transporter (CBT-1, NCU05853) and cellobionic acid phosphorylase (CAP, NCU09425). Extracellular cellobionic acid could be imported directly by CBT-1 or cleaved to gluconic acid and glucose by a β-glucosidase (NCU08755) outside the cells. Intracellular cellobionic acid was further cleaved to glucose 1-phosphate and gluconic acid by CAP. However, it remains unclear how N. crassa utilizes extracellular gluconic acid. The aldonic acid pathway was successfully implemented in Saccharomyces cerevisiae when N. crassa gluconokinase was co-expressed, resulting in cellobionic acid consumption in both aerobic and anaerobic conditions.
conclusionsWe successfully identified a branched aldonic acid utilization pathway in N. crassa and transferred its essential components into S. cerevisiae, a robust industrial microorganism.
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