Evidence map›Paper›PMID 26279644›Full record

ArticleJournal of the International Society of Sports Nutrition2015

Effects of oral phosphatidic acid feeding with or without whey protein on muscle protein synthesis and anabolic signaling in rodent skeletal muscle.

C Brooks Mobley, Troy A Hornberger, Carlton D Fox, James C Healy, Brian S Ferguson, Ryan P Lowery, Rachel M McNally, Christopher M Lockwood, Jeffrey R Stout, Andreas N Kavazis and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the International Society of Sports Nutrition, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 24 citations in OpenAlex.

  1. Review
  2. Review
  3. Molecular Regulation of Exercise-Induced Muscle Fiber Hypertrophy.Cold Spring Harbor perspectives in medicine · 2018
    Review
  4. Article
  5. Article
  6. "Nutraceuticals" in relation to human skeletal muscle and exercise.American journal of physiology. Endocrinology and metabolism · 2017
    Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 1 country.

C Brooks MobleySchool of Kinesiology, Auburn University, Auburn, AL USA.
Troy A HornbergerDepartment of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI USA.
Carlton D FoxSchool of Kinesiology, Auburn University, Auburn, AL USA.
James C HealySchool of Kinesiology, Auburn University, Auburn, AL USA.
Brian S FergusonSchool of Kinesiology, Auburn University, Auburn, AL USA.
Ryan P LoweryDepartment of Health Sciences and Human Performance, University of Tampa, Tampa, FL USA.
Rachel M McNallyDepartment of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI USA.
Christopher M Lockwood4LIFE Research, LLC, Sandy, UT USA.
Jeffrey R StoutHuman Performance Laboratory, University of Central Florida, Orlando, FL USA.
Andreas N KavazisSchool of Kinesiology, Auburn University, Auburn, AL USA.
Jacob M WilsonDepartment of Health Sciences and Human Performance, University of Tampa, Tampa, FL USA.
Michael D RobertsSchool of Kinesiology, Auburn University, Auburn, AL USA.
Auburn University · USUniversity of Tampa · USUniversity of Wisconsin–Madison · USUniversity of Central Florida · USWashburn University · US

Funding

Mechanotransduction and the Regulation of Skeletal Muscle MassR01AR057347 · NIAMS · UNIVERSITY OF WISCONSIN-MADISON · PI HORNBERGER, TROY A · 2010 to 2019
$3.6M
NIAMS NIH HHS AR057347NIAMS NIH HHS R01 AR057347
6 · The paper itself

Abstract

backgroundPhosphatidic acid (PA) is a diacyl-glycerophospholipid that acts as a signaling molecule in numerous cellular processes. Recently, PA has been proposed to stimulate skeletal muscle protein accretion, but mechanistic studies are lacking. Furthermore, it is unknown whether co-ingesting PA with other leucine-containing ingredients can enhance intramuscular anabolic signaling mechanisms. Thus, the purpose of this study was to examine if oral PA feeding acutely increases anabolic signaling markers and muscle protein synthesis (MPS) in gastrocnemius with and without whey protein concentrate (WPC).

methodsOvernight fasted male Wistar rats (~250 g) were randomly assigned to four groups: control (CON, n = 6-13), PA (29 mg; n = 8), WPC (197 mg; n = 8), or PA + WPC (n = 8). Three hours post-feeding, gastrocnemius muscle was removed for markers of Akt-mTOR signaling, gene expression patterns related to skeletal muscle mass regulation and metabolism, and MPS analysis via the SUnSET method.

resultsCompared to CON rats, PA, WPC and PA + WPC resulted in a significant elevation in the phosphorylation of mTOR (Ser2481) and rps6 (Ser235/236) (p < 0.05) in the gastrocnemius though there were no differences between the supplemented groups. MPS levels in the gastrocnemius were significantly (p < 0.05) elevated in WPC versus CON rats, and tended to be elevated in PA versus CON rats (p = 0.08), though MPS was less in PA + WPC versus WPC rats (p < 0.05) in spite of robust increases in mTOR pathway activity markers in the former group. C2C12 myoblast data agreed with the in vivo data herein showing that PA increased MPS levels 51% (p < 0.001) phosphorylated p70s6k (Thr389) levels 67% (p < 0.001).

conclusionsOur results are the first in vivo evidence to demonstrate that PA tends to increases MPS 3 h post-feeding, though PA may delay WPC-mediated MPS kinetics within a 3 h post-feeding window.

Indexed as

AnimalsBlood GlucoseGlucose Transporter Type 4MaleMechanistic Target of Rapamycin Complex 1Multiprotein ComplexesMuscle ProteinsMuscle, SkeletalPhosphatidic AcidsPhosphorylationProtein BiosynthesisProto-Oncogene Proteins c-aktRatsRats, WistarRibosomal Protein S6 Kinases, 70-kDaTOR Serine-Threonine KinasesBlood GlucoseGlucose Transporter Type 4Mechanistic Target of Rapamycin Complex 1Multiprotein ComplexesMuscle ProteinsPhosphatidic AcidsProto-Oncogene Proteins c-aktRibosomal Protein S6 Kinases, 70-kDaSlc2a4 protein, ratTOR Serine-Threonine KinasesWhey Proteins

Identifiers

PMID26279644
PMCPMC4537536
OpenAlexW2128673831

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.