ArticleChembiochem : a European journal of chemical biology2015
The Cremeomycin Biosynthetic Gene Cluster Encodes a Pathway for Diazo Formation.
Article in Chembiochem : a European journal of chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Microbial unusual gene clusters without prominent core enzymes: natural products, biosynthesis and genome mining.The Journal of antibiotics · 2026Review
- Role of a FAD-dependent monooxygenase in diazo group functionalization of kinamycin inMicrobiology (Reading, England) · 2025Article
- Recent Developments and Challenges in the Enzymatic Formation of Nitrogen-Nitrogen Bonds.ACS catalysis · 2025Review
- Genome Mining for Diazo-Synthesis-Related Genes inBiomolecules · 2024Article
- The shikimate pathway: gateway to metabolic diversity.Natural product reports · 2024Review
- Discovery of thiazostatin D/E using UPLC-HR-MS2-based metabolomics and σ-factor engineering ofFrontiers in bioengineering and biotechnology · 2024Article
- Discovery of the Azaserine Biosynthetic Pathway Uncovers a Biological Route for α-Diazoester Production.Angewandte Chemie (International ed. in English) · 2023Article
- Uncovering the Cryptic Gene ClusterInternational journal of molecular sciences · 2023Article
- Complete Genome Sequence of Two Deep-SeaMarine drugs · 2021Article
- Leveraging Microbial Genomes and Genomic Context for Chemical Discovery.Accounts of chemical research · 2021Article
- Flavin-dependent N-hydroxylating enzymes: distribution and application.Applied microbiology and biotechnology · 2020Review
- The l-Alanosine Gene Cluster Encodes a Pathway for Diazeniumdiolate Biosynthesis.Chembiochem : a European journal of chemical biology · 2020Article
- Identification of 8-Azaguanine Biosynthesis-Related Genes Provides Insight Into the Enzymatic and Non-enzymatic Biosynthetic Pathway for 1,2,3-Triazole.Frontiers in bioengineering and biotechnology · 2020Article
- Identifying the Biosynthetic Gene Cluster for Triacsins with an N-Hydroxytriazene Moiety.Chembiochem : a European journal of chemical biology · 2019Article
- Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis.The Journal of organic chemistry · 2018Article
- Heteroatom-Heteroatom Bond Formation in Natural Product Biosynthesis.Chemical reviews · 2017Review
- Cloning and Heterologous Expression of a Large-sized Natural Product Biosynthetic Gene Cluster inFrontiers in microbiology · 2017Review
- Diazo Compounds: Versatile Tools for Chemical Biology.ACS chemical biology · 2016Review
- A Mutasynthetic Library of Platensimycin and Platencin Analogues.Organic letters · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Diazo groups are found in a range of natural products that possess potent biological activities. Despite longstanding interest in these metabolites, diazo group biosynthesis is not well understood, in part because of difficulties in identifying specific genes linked to diazo formation. Here we describe the discovery of the gene cluster that produces the o-diazoquinone natural product cremeomycin and its heterologous expression in Streptomyces lividans. We used stable isotope feeding experiments and in vitro characterization of biosynthetic enzymes to decipher the order of events in this pathway and establish that diazo construction involves late-stage N-N bond formation. This work represents the first successful production of a diazo-containing metabolite in a heterologous host, experimentally linking a set of genes with diazo formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.