ArticleStructure (London, England : 1993)2015
A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.
Article in Structure (London, England : 1993), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Encoded Cell-Material Interactions to Reroute Cytokine Signaling for Regenerative Medicine.Advanced healthcare materials · 2026Article
- Encoded cell-material interactions to reroute cytokine signaling for regenerative medicine.bioRxiv : the preprint server for biology · 2025Article
- Structure-Based Understanding of CuBiosensors · 2025Review
- Engineering Rubisco condensation in chloroplasts to manipulate plant photosynthesis.Plant biotechnology journal · 2025Article
- Oligomerization Function of the Native Exon 5 Sequence of Ameloblastin Fused with Calmodulin.ACS omega · 2025Article
- Co-crystallization with diabodies: A case study for the introduction of synthetic symmetry.Structure (London, England : 1993) · 2021Article
- Designed Artificial Protein Heterodimers With Coupled Functions Constructed Using Bio-Orthogonal Chemistry.Frontiers in chemistry · 2021Article
- Article
- Development of imaging scaffolds for cryo-electron microscopy.Current opinion in structural biology · 2020Review
- Better together: building protein oligomers naturally and by design.Biochemical Society transactions · 2019Review
- Split Green Fluorescent Proteins: Scope, Limitations, and Outlook.Annual review of biophysics · 2019Review
- A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold.Nature communications · 2019Article
- Construction of supramolecular nanotubes from protein crystals.Chemical science · 2019Article
- Synthetic cytokine receptors transmit biological signals using artificial ligands.Nature communications · 2018Article
- Characterization of Split Fluorescent Protein Variants and Quantitative Analyses of Their Self-Assembly Process.Scientific reports · 2018Article
- Rewiring T-cell responses to soluble factors with chimeric antigen receptors.Nature chemical biology · 2018Article
- Native ESI Mass Spectrometry Can Help to Avoid Wrong Interpretations from Isothermal Titration Calorimetry in Difficult Situations.Journal of the American Society for Mass Spectrometry · 2017Article
- Crystal structure of the cyan fluorescent protein Cerulean-S175G.Acta crystallographica. Section F, Structural biology communications · 2016Article
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Authors and funding
7 authors.
Funding
Abstract
Applications ranging from synthetic biology to protein crystallization could be advanced by facile systems for connecting multiple proteins together in predefined spatial relationships. One approach to this goal is to engineer many distinct assembly forms of a single carrier protein or scaffold, to which other proteins of interest can then be readily attached. In this work we chose GFP as a scaffold and engineered many alternative oligomeric forms, driven by either specific disulfide bond formation or metal ion addition. We generated a wide range of spatial arrangements of GFP subunits from 11 different oligomeric variants, and determined their X-ray structures in a total of 33 distinct crystal forms. Some of the oligomeric GFP variants show geometric polymorphism depending on conditions, while others show considerable geometric rigidity. Potential future applications of this system are discussed.
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