ArticleAmerican journal of physiology. Heart and circulatory physiology2015
Mitochondrial flash as a novel biomarker of mitochondrial respiration in the heart.
Article in American journal of physiology. Heart and circulatory physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Matrix polyamines regulate bidirectional calcium flux through MCU.Structure (London, England : 1993) · 2026Article
- The mitochondrial CaScientific reports · 2022Article
- Energy Metabolism on Mitochondrial Maturation and Its Effects on Cardiomyocyte Cell Fate.Frontiers in cell and developmental biology · 2022Review
- Protocol for Measurement of Oxygen Consumption RateSTAR protocols · 2020Article
- Protocol for Imaging of Mitoflashes in Live Cardiomyocytes.STAR protocols · 2020Article
- Why don't mice lacking the mitochondrial CaThe Journal of physiology · 2020Review
- Integrating Ultra-Weak Photon Emission Analysis in Mitochondrial Research.Frontiers in physiology · 2020Review
- Mitochondrial Dynamics in Adult Cardiomyocytes and Heart Diseases.Frontiers in cell and developmental biology · 2020Review
- Mitochondrial CaFEBS letters · 2019Review
- Isolating a reverse-mode ATP synthase-dependent mechanism of mitoflash activation.The Journal of general physiology · 2019Article
- Mitoflash biogenesis and its role in the autoregulation of mitochondrial proton electrochemical potential.The Journal of general physiology · 2019Article
- Substrate-dependent and cyclophilin D-independent regulation of mitochondrial flashes in skeletal and cardiac muscle.Archives of biochemistry and biophysics · 2019Article
- SR-mitochondria communication in adult cardiomyocytes: A close relationship where the CaArchives of biochemistry and biophysics · 2019Review
- Skeletal muscle mitoflashes, pH, and the role of uncoupling protein-3.Archives of biochemistry and biophysics · 2019Article
- Dysregulated mitochondrial CaArchives of biochemistry and biophysics · 2019Review
- Heart specific knockout of Ndufs4 ameliorates ischemia reperfusion injury.Journal of molecular and cellular cardiology · 2018Article
- Ultrastructural Characterization of Flashing Mitochondria.Contact (Thousand Oaks (Ventura County, Calif.)) · 2018Article
- Regulation of mitochondrial bioenergetics by the non-canonical roles of mitochondrial dynamics proteins in the heart.Biochimica et biophysica acta. Molecular basis of disease · 2018Review
- Different approaches to modeling analysis of mitochondrial swelling.Mitochondrion · 2018Review
- Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Mitochondrial respiration through electron transport chain (ETC) activity generates ATP and reactive oxygen species in eukaryotic cells. The modulation of mitochondrial respiration in vivo or under physiological conditions remains elusive largely due to the lack of appropriate approach to monitor ETC activity in a real-time manner. Here, we show that ETC-coupled mitochondrial flash is a novel biomarker for monitoring mitochondrial respiration under pathophysiological conditions in cultured adult cardiac myocyte and perfused beating heart. Through real-time confocal imaging, we follow the frequency of a transient bursting fluorescent signal, named mitochondrial flash, from individual mitochondria within intact cells expressing a mitochondrial matrix-targeted probe, mt-cpYFP (mitochondrial-circularly permuted yellow fluorescent protein). This mt-cpYFP recorded mitochondrial flash has been shown to be composed of a major superoxide signal with a minor alkalization signal within the mitochondrial matrix. Through manipulating physiological substrates for mitochondrial respiration, we find a close coupling between flash frequency and the ETC electron flow, as measured by oxygen consumption rate in cardiac myocyte. Stimulating electron flow under physiological conditions increases flash frequency. On the other hand, partially block or slowdown electron flow by inhibiting the F0F1 ATPase, which represents a pathological condition, transiently increases then decreases flash frequency. Limiting electron entrance at complex I by knocking out Ndufs4, an assembling subunit of complex I, suppresses mitochondrial flash activity. These results suggest that mitochondrial electron flow can be monitored by real-time imaging of mitochondrial flash. The mitochondrial flash frequency could be used as a novel biomarker for mitochondrial respiration under physiological and pathological conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.