ArticleCell2015
Identification of Gene Positioning Factors Using High-Throughput Imaging Mapping.
Article in Cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
107 citing papers in PubMed.
- VPS4 and CHMP7 release centromeres from the nuclear envelope for post-mitotic positioning in daughter nuclei.bioRxiv : the preprint server for biology · 2026Article
- Re-establishment of TAD boundary organization during DNA replication.bioRxiv : the preprint server for biology · 2026Article
- Article
- Whole-genome 3D architectural screen reveals modulators of brain DNA structure.bioRxiv : the preprint server for biology · 2026Article
- The Biological Function of Genome Organization.International journal of molecular sciences · 2025Review
- Mechanistic and Epigenetic Partitioning of Lamina-Associated Chromatin Revealed by a Genome-Wide Imaging Screen.bioRxiv : the preprint server for biology · 2025Article
- Binding of Transcriptional Activator to Silent Loci Causes Their Detachment from the Nuclear Lamina inInternational journal of molecular sciences · 2025Article
- Perturb-tracing enables high-content screening of multi-scale 3D genome regulators.Nature methods · 2025Article
- Article
- High-throughput image processing software for the study of nuclear architecture and gene expression.Scientific reports · 2024Article
- Allele-level visualization of transcription and chromatin by high-throughput imaging.Histochemistry and cell biology · 2024Article
- OpenNucleome for high resolution nuclear structural and dynamical modeling.bioRxiv : the preprint server for biology · 2024Article
- Allele-level visualization of transcription and chromatin by high-throughput imaging.Research square · 2024Article
- Allele-level visualization of transcription and chromatin by high-throughput imaging.bioRxiv : the preprint server for biology · 2024Article
- Image-based 3D genomics through chromatin tracing.Nature reviews. Methods primers · 2024Article
- Article
- A spatial genome aligner for resolving chromatin architectures from multiplexed DNA FISH.Nature biotechnology · 2023Article
- 3D enhancer-promoter interactions and multi-connected hubs: Organizational principles and functional roles.Cell reports · 2023Review
- Dynamics of nuclear architecture during early embryonic development and lessons from liveimaging.Developmental cell · 2023Review
- (Tn5-)FISH-based imaging in the era of 3D/spatial genomics.Biophysics reports · 2023Article
47 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
Genomes are arranged non-randomly in the 3D space of the cell nucleus. Here, we have developed HIPMap, a high-precision, high-throughput, automated fluorescent in situ hybridization imaging pipeline, for mapping of the spatial location of genome regions at large scale. High-throughput imaging position mapping (HIPMap) enabled an unbiased siRNA screen for factors involved in genome organization in human cells. We identify 50 cellular factors required for proper positioning of a set of functionally diverse genomic loci. Positioning factors include chromatin remodelers, histone modifiers, and nuclear envelope and pore proteins. Components of the replication and post-replication chromatin re-assembly machinery are prominently represented among positioning factors, and timely progression of cells through replication, but not mitosis, is required for correct gene positioning. Our results establish a method for the large-scale mapping of genome locations and have led to the identification of a compendium of cellular factors involved in spatial genome organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.