ArticleCell2015
An Atypical AAA+ ATPase Assembly Controls Efficient Transposition through DNA Remodeling and Transposase Recruitment.
Article in Cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 43 citations in OpenAlex.
- Comparative structural analysis of IS21 transposition complexes using Cryo-EM and AlphaFold3.Mobile DNA · 2025Article
- Graph Attention Neural Networks Reveal TnsC Filament Assembly in a CRISPR-Associated Transposon.bioRxiv : the preprint server for biology · 2025Article
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- The gut microbiota modifies antibody durability and booster responses after SARS-CoV-2 vaccination.Journal of translational medicine · 2024Article
- Assembly of the Tn7 targeting complex by a regulated stepwise process.Molecular cell · 2024Article
- Article
- Mechanism of target site selection by type V-K CRISPR-associated transposases.Science (New York, N.Y.) · 2023Article
- IS21 family transposase cleaved donor complex traps two right-handed superhelical crossings.Nature communications · 2023Article
- Article
- Phylogenetically and catabolically diverse diazotrophs reside in deep-sea cold seep sediments.Nature communications · 2022Article
- Structural basis for DNA targeting by the Tn7 transposon.Nature structural & molecular biology · 2022Article
- Article
- Structural basis for target site selection in RNA-guided DNA transposition systems.Science (New York, N.Y.) · 2021Article
- The molecular coupling between substrate recognition and ATP turnover in a AAA+ hexameric helicase loader.eLife · 2021Article
- Diazotroph Genomes and Their Seasonal Dynamics in a Stratified Humic Bog Lake.Frontiers in microbiology · 2020Article
- Expanding the Diversity ofViruses · 2019Article
- Physical Basis for the Loading of a Bacterial Replicative Helicase onto DNA.Molecular cell · 2019Article
- Structural insights into the mechanism of double strand break formation by Hermes, a hAT family eukaryotic DNA transposase.Nucleic acids research · 2018Article
- Regulation of solvent tolerance in Pseudomonas putida S12 mediated by mobile elements.Microbial biotechnology · 2017Article
- Defining the crucial domain and amino acid residues in bacterial Lon protease for DNA binding and processing of DNA-interacting substrates.The Journal of biological chemistry · 2017Article
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2 authors at 1 institution in 1 country.
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Abstract
Transposons are ubiquitous genetic elements that drive genome rearrangements, evolution, and the spread of infectious disease and drug-resistance. Many transposons, such as Mu, Tn7, and IS21, require regulatory AAA+ ATPases for function. We use X-ray crystallography and cryo-electron microscopy to show that the ATPase subunit of IS21, IstB, assembles into a clamshell-shaped decamer that sandwiches DNA between two helical pentamers of ATP-associated AAA+ domains, sharply bending the duplex into a 180° U-turn. Biochemical studies corroborate key features of the structure and further show that the IS21 transposase, IstA, recognizes the IstB•DNA complex and promotes its disassembly by stimulating ATP hydrolysis. Collectively, these studies reveal a distinct manner of higher-order assembly and client engagement by a AAA+ ATPase and suggest a mechanistic model where IstB binding and subsequent DNA bending primes a selected insertion site for efficient transposition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.