ArticleReproductive biology and endocrinology : RB&E2015
Correlation of cumulus gene expression of GJA1, PRSS35, PTX3, and SERPINE2 with oocyte maturation, fertilization, and embryo development.
Article in Reproductive biology and endocrinology : RB&E, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 81 citations in OpenAlex.
- Biomarkers Assessing the Role of Cumulus Cells on IVF Outcomes: A Systematic Review.Journal of assisted reproduction and genetics · 2024Pooled it
- Is there a correlation between follicle size and gene expression in cumulus cells and is gene expression an indicator of embryo development?Reproductive biology and endocrinology : RB&E · 2018Trial
- Female Aging Affects Coilin Pattern in Mouse Cumulus Cells.Journal of developmental biology · 2026Article
- Furin-Mediated Cleavage of Zona Pellucida Proteins Is Essential for Oocyte Development.MedComm · 2025Article
- TRIB3 regulates Cx43 expression in human granulosa cells via the Wnt/β-catenin signaling pathway: an in vitro study.Journal of ovarian research · 2025Article
- Exploring the Potential Impact of SERPINE Gene Expression in Cumulus Cells During Fertility Treatments: A Single Center Study.Clinics and practice · 2025Article
- Effect of Bovine Follicular Fluid Small Extracellular Vesicles Isolated by Ultracentrifugation and Chromatography on In Vitro Oocyte Maturation and Embryo Development.International journal of molecular sciences · 2025Article
- Identification of functional circRNAs regulating ovarian follicle development in goats.BMC genomics · 2024Article
- Maturational competence of equine oocytes is associated with alterations in their 'cumulome'.Molecular human reproduction · 2024Article
- Current Status of Ovarian and Endometrial Biomarkers in Predicting ART Outcomes.Journal of clinical medicine · 2024Review
- New Approach to the Cryopreservation of GV Oocytes and Cumulus Cells through the Lens of Preserving the Intercellular Gap Junctions Based on the Bovine Model.International journal of molecular sciences · 2024Article
- Influence of Long Non-Coding RNAs on Human Oocyte Development.Pharmacogenomics and personalized medicine · 2024Review
- Cyclic AMP mediates ovine cumulus-oocyte gap junctional function via balancing connexin 43 expression and phosphorylation.Endocrine connections · 2023Article
- Secreted protease PRSS35 suppresses hepatocellular carcinoma by disabling CXCL2-mediated neutrophil extracellular traps.Nature communications · 2023Article
- Evaluation of the expression profile of mRNAs and lncRNAs in cumulus cells associated with polycystic ovary syndrome and pregnancy.Iranian journal of basic medical sciences · 2023Article
- Potential biomarkers to predict return to fertility after discontinuation of female contraceptives-looking to the future.Frontiers in reproductive health · 2023Review
- Application of Single-Cell RNA Sequencing in Ovarian Development.Biomolecules · 2022Review
- A single-cell atlas of the cycling murine ovary.eLife · 2022Article
- The transcriptome-wide N6-methyladenosine (mBMC genomics · 2022Article
- Molecular Signatures Correlated With Poor IVF Outcomes: Insights From the mRNA and lncRNA Expression of Endometriotic Granulosa Cells.Frontiers in endocrinology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGJA1 and PTX3 were proposed as gene markers for oocyte and embryo developmental competence, while SERPINE2 was reported to be associated with pregnancy outcome. PRSS35, which is exclusively expressed in the ovary, may be correlated with oocyte competence. This study was conducted to evaluate the correlation of cumulus GJA1, PRSS35, PTX3, and SERPINE2 gene expression levels with oocyte maturation, fertilization, and early embryo development.
methodsIn total, 308 cumulus cell samples separated from individual cumulus-oocyte complex were obtained from 40 patients undergoing the intracytoplasmic sperm injection treatment procedure. Gene expression levels (mRNA levels) in cumulus cells were assessed using quantitative real-time polymerase chain reaction.
resultsGene expression levels of GJA1 and SERPINE2 in cumulus cells surrounding mature oocytes were significantly lower than those in cumulus cells enclosing immature oocytes. PRSS35 mRNA levels in cumulus cells of fertilized oocytes were significantly higher than those in cumulus cells of unfertilized oocytes. GJA1 and SERPINE2 seemed to express higher mRNA levels, while PRSS35 showed lower expression in cumulus cells of oocytes that developed into embryos with good morphology; however, the expression levels of all three genes and PTX3 showed no significant differences between embryos with good or poor morphology.
conclusionsGJA1 and SERPINE2 represent potential gene markers associated with oocyte maturation. PRSS35 may be correlated with oocyte fertilization potential. However, GJA1, PRSS35, PTX3, and SERPINE2 may not be considered as marker genes for predicting embryo morphology.
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