ArticleBMC veterinary research2015
The major acute phase proteins of bovine milk in a commercial dairy herd.
Article in BMC veterinary research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.
- Milk proteins as mastitis markers in dairy ruminants - a systematic review.Veterinary research communications · 2022Pooled it
- Inclusion of Curcumin in the Diet of Prepartum Cows and Its Effects on Animal Health and Production.Veterinary sciences · 2026Article
- Association of Milk C-Reactive Protein With Subclinical Mastitis in Dairy Buffaloes.Veterinary medicine and science · 2026Article
- Comprehensive evaluation of milk biomarkers as indicators of intramammary infection in dairy goats across lactation.Scientific reports · 2026Article
- Functional Role and Diagnostic Potential of Biomarkers in the Early Detection of Mastitis in Dairy Cows.Animals : an open access journal from MDPI · 2026Review
- Biomarkers for subclinical bovine mastitis: a high throughput TMT-based proteomic investigation.Veterinary research communications · 2024Article
- Novel Approach for Evaluating Pregnancy-Associated Glycoprotein and Inflammation Markers during the Postpartum Period in Holstein Friesian Cows.Animals : an open access journal from MDPI · 2024Article
- Evaluation of Recombinant Bovine Interleukin-8 (rbIL-8) as a Treatment for Chronic Intramammary Infection in Dairy Cows.Antibiotics (Basel, Switzerland) · 2022Article
- Can Procalcitonin Be Dosed in Bovine Milk Using a Commercial ELISA Kit?Animals : an open access journal from MDPI · 2022Article
- Facile construction of a molecularly imprinted polymer-based electrochemical sensor for the detection of milk amyloid A.Mikrochimica acta · 2020Article
- Concentrations of Acute-Phase Proteins in Milk from Cows with Clinical Mastitis Caused by Different Pathogens.Pathogens (Basel, Switzerland) · 2020Article
- Differential expression of serum amyloid A1 and A3 in bovine epithelia.The Journal of veterinary medical science · 2020Article
- Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci.Scientific reports · 2019Article
- Calibration of Novel Protein Biomarkers for Veterinary Clinical Pathology: A Call for International Action.Frontiers in veterinary science · 2019Review
- Article
- Study on the accuracy of milk amyloid A test and other diagnostic methods for identification of milk quality.Veterinary research forum : an international quarterly journal · 2018Article
- Identification of Host Defense-Related Proteins Using Label-Free Quantitative Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus Subclinical Mastitis.International journal of molecular sciences · 2017Article
- The anti-inflammatory and antioxidant effects of melatonin on LPS-stimulated bovine mammary epithelial cells.PloS one · 2017Article
- Acute phase response in lame crossbred dairy cattle.Veterinary world · 2016Article
- Characterization of Haptoglobin Isotype in Milk of Mastitis-Affected Cows.Veterinary sciences · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMilk acute phase proteins (APP) have been identified and show promise as biomarkers of mastitis. However analysis of their profile in dairy cows from a production herd is necessary in order to confirm their benefits in mastitis diagnosis. The profiles of milk haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) were determined in 54 composite milk (milk from all functional quarters of a cow's udder collected in a common receptacle) samples (CMS) from a commercial dairy farm. Milk Hp was also determined in individual quarter milk (milk from a single udder quarter) samples (QMS) (n = 149) of the cows. An ELISA was developed and validated for the determination of milk Hp while commercial kits were used for M-SAA3 and CRP assay respectively. Composite milk APP results were compared with cow factors including parity, stage of lactation, percentage protein and fat as well as somatic cell counts (SCC).
resultsComposite milk Hp ranged from <0.4-55 μg/ml with a median of 3.5 μg/ml; composite milk M-SAA3 ranged from <0.6-50 μg/ml and had a median of 1.2 μg/ml, while CRP ranged from <1.80-173 ng/ml and had a median of 24.6 ng/ml. Significant correlations were found between composite SCC and Hp (P-value <0.009) as well as parity and Hp (P < 0.009), but not between M-SAA3 and SCC, M-SAA3 and Hp, M-SAA3 and CRP or M-SAA3 and parity. Milk CRP was correlated with % fat (P = 0.002) and % protein (P = 0.001) of the milk samples. The lack of correlation of SCC with the M-SAA3 and CRP could result from these APP being more sensitive to intra-mammary infection than SCC. Quarter milk Hp had a range of <0.4-420 μg/ml with a median value of 3.6 μg/ml, with 92 % of samples below 20 μg/ml.
conclusionBaseline values of Hp, M-SAA3 and CRP were established in composite milk from cows with normal SCC on the dairy farm. Parity was recognized as a possible confounding factor when diagnosing mastitis using Hp. The value of the APP, Hp, M-SAA3 and CRP as substitutes or to complement SCC in indicating udder inflammation, was demonstrated.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.