Evidence map›Paper›PMID 26276431›Full record

ArticleJournal of assisted reproduction and genetics2015

Ultrastructural markers of quality are impaired in human metaphase II aged oocytes: a comparison between reproductive and in vitro aging.

S Bianchi, G Macchiarelli, G Micara, A Linari, C Boninsegna, C Aragona, G Rossi, S Cecconi, S A Nottola

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed, 3 pooled it
5.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 3 syntheses or guidelines pooled it, 76 citations in OpenAlex.

  1. Pooled it
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  3. Association between Female Reproductive Health and Mancozeb: Systematic Review of Experimental Models.International journal of environmental research and public health · 2020
    Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

S BianchiDepartment of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
G MacchiarelliDepartment of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
G MicaraDepartment of Gynaecology-Obstetrics & Urology, "Sapienza" University, Rome, Italy.
A LinariDepartment of Gynaecology-Obstetrics & Urology, "Sapienza" University, Rome, Italy.
C BoninsegnaDepartment of Gynaecology-Obstetrics & Urology, "Sapienza" University, Rome, Italy.
C AragonaDepartment of Gynaecology-Obstetrics & Urology, "Sapienza" University, Rome, Italy.
G RossiDepartment of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
S CecconiDepartment of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
S A NottolaDepartment of Anatomy, Histology, Forensic Medicine and Orthopaedics, "Sapienza" University, Rome, Italy. stefania.nottola@uniroma1.it.
Sapienza University of Rome · ITUniversity of L'Aquila · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeChildbearing delay contributes to the increase of subfertile couples that require assisted reproductive technology (ART). Subfertility relates with reproductive aging (RA). In vitro aging (IvA) (due to extended culture) may also impair oocyte competence. Aims of this study were to evaluate and compare the oocyte ultrastructure after RA and IvA.

methodsCumulus-oocyte complexes (COCs) (n = 68), with metaphase II oocyte and expanded cumulus, from consenting patients (<35 years old and ≥35 years old, n = 36), were selected by phase contrast microscopy and fixed at pick up, or after 24 h culture. COCs (n = 44) were studied by light and qualitative/morphometric transmission electron microscopy. Two-way ANOVA, with age and culture as grouping factors, was applied for statistical analysis (p < 0.05). Metaphase II cumulus-free oocytes (n = 24) were selected for confocal microscopy observations.

resultsSignificant decrease of mitochondria-smooth endoplasmic reticulum aggregates, increase of mitochondria-vesicle complexes size and amount, decrease of cortical granules and microvilli, and alterations of the spindle structure characterized both RA and IvA oocytes. These changes were significantly more evident in the RA oocytes submitted to IvA. RA oocytes also showed changes of the zona pellucida and occurrence of vacuoles after culture. Cumuli appeared re-compacted after culture, irrespective of the age of the patients.

conclusionsThese data demonstrated that aging is related to decay of oocyte ultrastructural quality, and that oocytes from elder women are more sensitive to prolonged culture (IvA) than the oocytes from younger women. These morphological results should be considered when applying ART in aged patients, rescue ICSI, or artificial oocyte activation.

Indexed as

AdultAgingBiomarkersCumulus CellsFemaleHumansMeiosisMetaphaseMicroscopy, ConfocalMicroscopy, ElectronOocytesReproductionReproductive Techniques, AssistedSpindle ApparatusZona PellucidaBiomarkersAgingAssisted reproductionHumanOocyteQualityUltrastructure

Identifiers

PMID26276431
PMCPMC4595403
OpenAlexW1445448757

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.