ArticlePflugers Archiv : European journal of physiology2015
UCP2 modulates single-channel properties of a MCU-dependent Ca(2+) inward current in mitochondria.
Article in Pflugers Archiv : European journal of physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Reduced Cerebral Infarct Volume in Young UCP2Cells · 2026Article
- Emerging role and the signaling pathways of uncoupling protein 2 in kidney diseases.Renal failure · 2024Review
- Post-translational modifications and protein quality control of mitochondrial channels and transporters.Frontiers in cell and developmental biology · 2023Review
- Article
- Cell death regulation by MAMs: from molecular mechanisms to therapeutic implications in cardiovascular diseases.Cell death & disease · 2022Review
- Mitochondrial calcium exchange in physiology and disease.Physiological reviews · 2022Review
- Mitochondrial CaInternational journal of molecular sciences · 2022Review
- Mitochondria-Associated Endoplasmic Reticulum Membranes in Cardiovascular Diseases.Frontiers in cell and developmental biology · 2020Review
- Pathophysiology of Calcium Mediated Ventricular Arrhythmias and Novel Therapeutic Options with Focus on Gene Therapy.International journal of molecular sciences · 2019Review
- Mitochondrial Calcium Uniporter Structure and Function in Different Types of Muscle Tissues in Health and Disease.International journal of molecular sciences · 2019Review
- Mitochondrial Uncoupling Proteins: Subtle Regulators of Cellular Redox Signaling.Antioxidants & redox signaling · 2018Review
- Increased mitochondrial calcium uniporter in adipocytes underlies mitochondrial alterations associated with insulin resistance.American journal of physiology. Endocrinology and metabolism · 2017Article
- DNP, mitochondrial uncoupling, and neuroprotection: A little dab'll do ya.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2017Article
- Intact mitochondrial CaFree radical biology & medicine · 2017Article
- Computational properties of mitochondria in T cell activation and fate.Open biology · 2016Review
- PRMT1-mediated methylation of MICU1 determines the UCP2/3 dependency of mitochondrial Ca(2+) uptake in immortalized cells.Nature communications · 2016Article
- UCP3 Regulates Single-Channel Activity of the Cardiac mCa1.The Journal of membrane biology · 2016Article
- The mitochondrial Ca2+ uniporter: regulation by auxiliary subunits and signal transduction pathways.American journal of physiology. Cell physiology · 2016Review
- Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The mitochondrial Ca(2+) uniporter is a highly Ca(2+)-selective protein complex that consists of the pore-forming mitochondrial Ca(2+) uniporter protein (MCU), the scaffolding essential MCU regulator (EMRE), and mitochondrial calcium uptake 1 and 2 (MICU1/2), which negatively regulate mitochondrial Ca(2+) uptake. We have previously reported that uncoupling proteins 2 and 3 (UCP2/3) are also engaged in the activity of mitochondrial Ca(2+) uptake under certain conditions, while the mechanism by which UCP2/3 facilitates mitochondrial Ca(2+) uniport remains elusive. This work was designed to investigate the impact of UCP2 on the three distinct mitochondrial Ca(2+) currents found in mitoplasts isolated from HeLa cells, the intermediate- (i-), burst- (b-) and extra-large (xl-) mitochondrial/mitoplast Ca(2+) currents (MCC). Using the patch clamp technique on mitoplasts from cells with reduced MCU and EMRE unveiled a very high affinity of MCU for xl-MCC that succeeds that for i-MCC, indicating the coexistence of at least two MCU/EMRE-dependent Ca(2+) currents. The manipulation of the expression level of UCP2 by either siRNA-mediated knockdown or overexpression changed exclusively the open probability (NPo) of xl-MCC by approx. 38% decrease or nearly a 3-fold increase, respectively. These findings confirm a regulatory role of UCP2 in mitochondrial Ca(2+) uptake and identify UCP2 as a selective modulator of just one distinct MCU/EMRE-dependent mitochondrial Ca(2+) inward current.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.