Evidence map›Paper›PMID 26274878›Full record

ArticleJournal of visualized experiments : JoVE2015

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles.

Paola L Carvajal Monroy, Zipora Yablonka-Reuveni, Sander Grefte, Anne Marie Kuijpers-Jagtman, Frank A D T G Wagener, Johannes W Von den Hoff

Open access · greenAbstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.5field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Paola L Carvajal MonroyDepartment of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center.
Zipora Yablonka-ReuveniDepartment of Biological Structure, University of Washington School of Medicine.
Sander GrefteDepartment of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center.
Anne Marie Kuijpers-JagtmanDepartment of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center.
Frank A D T G WagenerDepartment of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center.
Johannes W Von den HoffDepartment of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center; hans.vondenhoff@radboudumc.nl.
Radboud University Nijmegen · NLUniversity of Washington · US

Funding

Myogenic Stem Cell Function in Aging Skeletal MuscleR01AG021566 · NIA · UNIVERSITY OF WASHINGTON · PI YABLONKA-REUVENI, ZIPORA · 2002 to 2012
$3.6M
Myogenic stem cells in extraocular musclesR01AG035377 · NIA · UNIVERSITY OF WASHINGTON · PI YABLONKA-REUVENI, ZIPORA · 2009 to 2014
$1.6M
Can klotho alleviate muscle fibrosis in muscular dystrophy?R21NS090051 · NINDS · UNIVERSITY OF WASHINGTON · PI YABLONKA-REUVENI, ZIPORA · 2014 to 2015
$425k
NIA NIH HHS AG021566NIA NIH HHS AG035377NIA NIH HHS R01 AG021566NIA NIH HHS R01 AG035377NINDS NIH HHS NS090051NINDS NIH HHS R21 NS090051
6 · The paper itself

Abstract

Fibrosis and defective muscle regeneration can hamper the functional recovery of the soft palate muscles after cleft palate repair. This causes persistent problems in speech, swallowing, and sucking. In vitro culture systems that allow the study of satellite cells (myogenic stem cells) from head muscles are crucial to develop new therapies based on tissue engineering to promote muscle regeneration after surgery. These systems will offer new perspectives for the treatment of cleft palate patients. A protocol for the isolation, culture and differentiation of satellite cells from head muscles is presented. The isolation is based on enzymatic digestion and trituration to release the satellite cells. In addition, this protocol comprises an innovative method using extracellular matrix gel coatings of millimeter size, which requires only low numbers of satellite cells for differentiation assays.

Indexed as

RegenerationAnimalsCell DifferentiationCell SeparationCleft PalateHeadPalate, SoftPharyngeal MusclesRatsSatellite Cells, Skeletal Muscle

Identifiers

PMID26274878
PMCPMC4544364
OpenAlexW1560040948

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.