ArticleJournal of visualized experiments : JoVE2015
Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The trial behind it
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Who cites it
25 citing papers in PubMed, 35 citations in OpenAlex.
- Hydrogen evolution and dynamics in hydrogel electrochemical cells for ischemia-reperfusion therapy.Nature chemical engineering · 2025Article
- Gold-modified nanoporous silicon for photoelectrochemical regulation of intracellular condensates.Nature nanotechnology · 2025Article
- Modified Langendorff Perfusion Method for Extended Perfusion Times of Rodent Cardiac Grafts.Journal of visualized experiments : JoVE · 2024Article
- Heart immunoengineering by lentiviral vector-mediated genetic modification during normothermicFrontiers in immunology · 2024Article
- Long-term inorganic nitrate administration protects against myocardial ischemia-reperfusion injury in female rats.BMC cardiovascular disorders · 2023Article
- Long-term Ovariectomy Reduces Tolerance of Rats to Myocardial Ischemia-reperfusion Injury.International journal of endocrinology and metabolism · 2023Article
- Intra-ischemic hypothermia cardioprotection involves modulation of PTEN/Akt/ERK signaling and fatty acid oxidation.Physiological reports · 2023Article
- Cardioprotective action of aprepitant in a rat model of ischemia-reperfusioninduced myocardial injury: role of PI3K-AkT-GSK-3β-HIF-1α signaling pathway.Acta cirurgica brasileira · 2022Article
- Cardiac phospholipidome is altered during ischemia and reperfusion in an ex vivo rat model.Biochemistry and biophysics reports · 2021Article
- Cannabidiol-mediated RISK PI3K/AKT and MAPK/ERK pathways decreasing reperfusion myocardial damage.Pharmacology research & perspectives · 2021Article
- Sweroside Protects Against Myocardial Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress and Pyroptosis Partially via Modulation of the Keap1/Nrf2 Axis.Frontiers in cardiovascular medicine · 2021Article
- miRNA-27a Transcription Activated by c-Fos Regulates Myocardial Ischemia-Reperfusion Injury by Targeting ATAD3a.Oxidative medicine and cellular longevity · 2021Article
- Protective effect of dimethyl fumarate on oxidative damage and signaling in cardiomyocytes.Molecular medicine reports · 2020Article
- Changes in nitric oxide synthase levels are associated with impaired cardiac function and tolerance to ischemia-reperfusion injury in male rats with transient congenital hypothyroidism.Naunyn-Schmiedeberg's archives of pharmacology · 2020Article
- Curculigoside attenuates myocardial ischemia‑reperfusion injury by inhibiting the opening of the mitochondrial permeability transition pore.International journal of molecular medicine · 2020Article
- Sappanone A Protects Against Myocardial Ischemia Reperfusion Injury by Modulation of Nrf2.Drug design, development and therapy · 2020Article
- Down-regulation of GAS5 ameliorates myocardial ischaemia/reperfusion injury via the miR-335/ROCK1/AKT/GSK-3β axis.Journal of cellular and molecular medicine · 2019Article
- Rosuvastatin Attenuates Myocardial Ischemia-Reperfusion Injury via UpregulatingCellular reprogramming · 2019Article
- Investigating the physiology of normothermic ex vivo heart perfusion in an isolated slaughterhouse porcine model used for device testing and training.BMC cardiovascular disorders · 2019Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The biochemical events surrounding ischemia reperfusion injury in the acute setting are of great importance to furthering novel treatment options for myocardial infarction and cardiac complications of thoracic surgery. The ability of certain drugs to precondition the myocardium against ischemia reperfusion injury has led to multiple clinical trials, with little success. The isolated heart model allows acute observation of the functional effects of ischemia reperfusion injury in real time, including the effects of various pharmacological interventions administered at any time-point before or within the ischemia-reperfusion injury window. Since brief periods of ischemia can precondition the heart against ischemic injury, in situ aortic cannulation is performed to allow for functional assessment of non-preconditioned myocardium. A saline filled balloon is placed into the left ventricle to allow for real-time measurement of pressure generation. Ischemic injury is simulated by the cessation of perfusion buffer flow, followed by reperfusion. The duration of both ischemia and reperfusion can be modulated to examine biochemical events at any given time-point. Although the Langendorff isolated heart model does not allow for the consideration of systemic events affecting ischemia and reperfusion, it is an excellent model for the examination of acute functional and biochemical events within the window of ischemia reperfusion injury as well as the effect of pharmacological intervention on cardiac pre- and postconditioning. The goal of this protocol is to demonstrate how to perform in situ aortic cannulation and heart excision followed by ischemia/reperfusion injury in the Langendorff model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.