ArticleeLife2015
A high affinity RIM-binding protein/Aplip1 interaction prevents the formation of ectopic axonal active zones.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- Blobby is a synaptic active zone assembly protein required for memory in Drosophila.Nature communications · 2025Article
- Synaptic Physiology Depends on Electrical Forces and Liquid-Liquid Phase Separation.Reviews of physiology, biochemistry and pharmacology · 2025Review
- Presynaptic perspective: Axonal transport defects in neurodevelopmental disorders.The Journal of cell biology · 2024Review
- Presynaptic Precursor Vesicles-Cargo, Biogenesis, and Kinesin-Based Transport across Species.Cells · 2023Review
- Module walking using an SH3-like cell-wall-binding domain leads to a new GH184 family of muramidases.Acta crystallographica. Section D, Structural biology · 2023Article
- Interactive nanocluster compaction of the ELKS scaffold and Cacophony CaScience advances · 2023Article
- Rab2 regulates presynaptic precursor vesicle biogenesis at the trans-Golgi.The Journal of cell biology · 2021Article
- RIM-binding protein couples synaptic vesicle recruitment to release sites.The Journal of cell biology · 2020Article
- Synaptic ribbons foster active zone stability and illumination-dependent active zone enrichment of RIM2 and Cav1.4 in photoreceptor synapses.Scientific reports · 2020Article
- RIMB-1/RIM-Binding Protein and UNC-10/RIM Redundantly Regulate Presynaptic Localization of the Voltage-Gated Calcium Channel inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Rapid active zone remodeling consolidates presynaptic potentiation.Nature communications · 2019Article
- Vertebrate Presynaptic Active Zone Assembly: a Role Accomplished by Diverse Molecular and Cellular Mechanisms.Molecular neurobiology · 2018Review
- Active zone proteins are transported via distinct mechanisms regulated by Par-1 kinase.PLoS genetics · 2017Article
- Synaptic Vesicle Proteins and Active Zone Plasticity.Frontiers in synaptic neuroscience · 2016Article
Corrections and comments
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Authors and funding
20 authors at 4 institutions in 2 countries.
Funding
Abstract
Synaptic vesicles (SVs) fuse at active zones (AZs) covered by a protein scaffold, at Drosophila synapses comprised of ELKS family member Bruchpilot (BRP) and RIM-binding protein (RBP). We here demonstrate axonal co-transport of BRP and RBP using intravital live imaging, with both proteins co-accumulating in axonal aggregates of several transport mutants. RBP, via its C-terminal Src-homology 3 (SH3) domains, binds Aplip1/JIP1, a transport adaptor involved in kinesin-dependent SV transport. We show in atomic detail that RBP C-terminal SH3 domains bind a proline-rich (PxxP) motif of Aplip1/JIP1 with submicromolar affinity. Pointmutating this PxxP motif provoked formation of ectopic AZ-like structures at axonal membranes. Direct interactions between AZ proteins and transport adaptors seem to provide complex avidity and shield synaptic interaction surfaces of pre-assembled scaffold protein transport complexes, thus, favouring physiological synaptic AZ assembly over premature assembly at axonal membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.