ArticlePloS one2015
Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A Quantitative Real-Time PCR Assay for Detection and Quantification of the Ginseng Alternaria Leaf and Stem Blight PathogenJournal of fungi (Basel, Switzerland) · 2026Article
- A qPCR assay for the detection of Phytophthora abietivora, an emerging pathogen on fir species cultivated as Christmas trees.PloS one · 2025Article
- Article
- A rapid LAMP assay for the diagnosis of oak wilt with the naked eye.Plant methods · 2024Article
- Thousand Cankers Disease in Walnut Trees in Europe: Current Status and Management.Pathogens (Basel, Switzerland) · 2023Review
- Loop-Mediated Isothermal Amplification (LAMP) and SYBR Green qPCR for Fast and Reliable Detection ofPlants (Basel, Switzerland) · 2022Article
- Rapid Detection ofPlants (Basel, Switzerland) · 2021Article
- Geosmithia associated with hardwood-infesting bark and ambrosia beetles, with the description of three new species from Poland.Antonie van Leeuwenhoek · 2021Article
- Application of Real-Time PCR for the Detection and Quantification of Oomycetes in Ornamental Nursery Stock.Journal of fungi (Basel, Switzerland) · 2021Article
- Fast and reliable molecular methods to detect fungal pathogens in woody plants.Applied microbiology and biotechnology · 2020Review
- Genomic biosurveillance of forest invasive alien enemies: A story written in code.Evolutionary applications · 2020Review
- A duplex real-time PCR with probe for simultaneous detection of Geosmithia morbida and its vector Pityophthorus juglandis.PloS one · 2020Article
- In Situ Processing and Efficient Environmental Detection (iSPEED) of tree pests and pathogens using point-of-use real-time PCR.PloS one · 2020Article
- Transferability of PCR-based diagnostic protocols: An international collaborative case study assessing protocols targeting the quarantine pine pathogen Fusarium circinatum.Scientific reports · 2019Article
- Pest categorisation ofEFSA journal. European Food Safety Authority · 2018Article
- Article
- A novel molecular toolkit for rapid detection of the pathogen and primary vector of thousand cankers disease.PloS one · 2018Article
- Genome sequences of sixGenomics data · 2016Article
- Scaling up discovery of hidden diversity in fungi: impacts of barcoding approaches.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Invasive alien tree pathogens can cause significant economic losses as well as large-scale damage to natural ecosystems. Early detection to prevent their establishment and spread is an important approach used by several national plant protection organizations (NPPOs). Molecular detection tools targeting 10 of the most unwanted alien forest pathogens in Canada were developed as part of the TAIGA project (http://taigaforesthealth.com/). Forest pathogens were selected following an independent prioritization. Specific TaqMan real-time PCR detection assays were designed to function under homogeneous conditions so that they may be used in 96- or 384-well plate format arrays for high-throughput testing of large numbers of samples against multiple targets. Assays were validated for 1) specificity, 2) sensitivity, 3) precision, and 4) robustness on environmental samples. All assays were highly specific when evaluated against a panel of pure cultures of target and phylogenetically closely-related species. Sensitivity, evaluated by assessing the limit of detection (with a threshold of 95% of positive samples), was found to be between one and ten target gene region copies. Precision or repeatability of each assay revealed a mean coefficient of variation of 3.4%. All assays successfully allowed detection of target pathogen on positive environmental samples, without any non-specific amplification. These molecular detection tools will allow for rapid and reliable detection of 10 of the most unwanted alien forest pathogens in Canada.
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