ArticlePloS one2015
Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 70 citations in OpenAlex.
- A novel culture flask for clinostat-based simulation of extraterrestrial gravities.Frontiers in cell and developmental biology · 2026Article
- Lipidomics of Caco-2 Cells Under Simulated Microgravity Conditions.International journal of molecular sciences · 2024Article
- Pharmacological Innovations in Space: Challenges and Future Perspectives.Pharmaceutical research · 2024Review
- Omics Studies of Specialized Cells and Stem Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Omics Studies of Tumor Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Technology using simulated microgravity.Regenerative therapy · 2023Review
- Fluid and Bubble Flow Detach Adherent Cancer Cells to Form Spheroids on a Random Positioning Machine.Cells · 2023Article
- Customized small-sized clinostat using 3D printing and gas-permeable polydimethylsiloxane culture dish.NPJ microgravity · 2023Article
- Dexamethasone Selectively Inhibits Detachment of Metastatic Thyroid Cancer Cells during Random Positioning.Cancers · 2023Article
- Lab-on-a-Chip Technologies for Microgravity Simulation and Space Applications.Micromachines · 2022Review
- RCCS Bioreactor-Based Modeled Microgravity Affects Gastric Cancer Cells and Improves the Chemotherapeutic Effect.Membranes · 2022Article
- The effects of microgravity on bone structure and function.NPJ microgravity · 2022Review
- The Fight against Cancer by Microgravity: The Multicellular Spheroid as a Metastasis Model.International journal of molecular sciences · 2022Review
- Three-Dimensional Growth of Prostate Cancer Cells Exposed to Simulated Microgravity.Frontiers in cell and developmental biology · 2022Article
- Simulating microgravity using a random positioning machine for inducing cellular responses to mechanotransduction in human osteoblasts.The Review of scientific instruments · 2021Article
- The CellBox-2 Mission to the International Space Station: Thyroid Cancer Cells in Space.International journal of molecular sciences · 2021Article
- Cell and Molecular Biology of Thyroid Disorders 2.0.International journal of molecular sciences · 2021Article
- Testing Lab-on-a-Chip Technology for Culturing Human Melanoma Cells under Simulated Microgravity.Cancers · 2021Article
- Survival Pathways Are Differently Affected by Microgravity in Normal and Cancerous Breast Cells.International journal of molecular sciences · 2021Article
- Alterations of Growth and Focal Adhesion Molecules in Human Breast Cancer Cells Exposed to the Random Positioning Machine.Frontiers in cell and developmental biology · 2021Article
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Authors and funding
15 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we focused on gravity-sensitive proteins of two human thyroid cancer cell lines (ML-1; RO82-W-1), which were exposed to a 2D clinostat (CLINO), a random positioning machine (RPM) and to normal 1g-conditions. After a three (3d)- or seven-day-culture (7d) on the two devices, we found both cell types growing three-dimensionally within multicellular spheroids (MCS) and also cells remaining adherent (AD) to the culture flask, while 1g-control cultures only formed adherent monolayers, unless the bottom of the culture dish was covered by agarose. In this case, the cytokines IL-6 and IL-8 facilitated the formation of MCS in both cell lines using the liquid-overlay technique at 1g. ML-1 cells grown on the RPM or the CLINO released amounts of IL-6 and MCP-1 into the supernatant, which were significantly elevated as compared to 1g-controls. Release of IL-4, IL-7, IL-8, IL-17, eotaxin-1 and VEGF increased time-dependently, but was not significantly influenced by the gravity conditions. After 3d on the RPM or the CLINO, an accumulation of F-actin around the cellular membrane was detectable in AD cells of both cell lines. IL-6 and IL-8 stimulation of ML-1 cells for 3d and 7d influenced the protein contents of ß1-integrin, talin-1, Ki-67, and beta-actin dose-dependently in adherent cells. The ß1-integrin content was significantly decreased in AD and MCS samples compared with 1g, while talin-1 was higher expressed in MCS than AD populations. The proliferation marker Ki-67 was elevated in AD samples compared with 1g and MCS samples. The ß-actin content of R082-W-1 cells remained unchanged. ML-1 cells exhibited no change in ß-actin in RPM cultures, but a reduction in CLINO samples. Thus, we concluded that simulated microgravity influences the release of cytokines in follicular thyroid cancer cells, and the production of ß1-integrin and talin-1 and predicts an identical effect under real microgravity conditions.
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