ArticleCell systems2015
Functional hierarchy of redundant actin assembly factors revealed by fine-grained registration of intrinsic image fluctuations.
Article in Cell systems, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
50 citing papers in PubMed, 80 citations in OpenAlex.
- Integrins and tetraspanins mediate CD36-actin coupling and regulate CD36 organization and signaling.The Journal of cell biology · 2026Article
- ERK builds a population of short-lived nascent adhesions that produce persistent edge protrusion and cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Allosteric Biosensors Unravel GTPase-Effector Feedback.bioRxiv : the preprint server for biology · 2026Article
- Reconstructing Actin Dynamics of the Leading Edge from Observational Data.bioRxiv : the preprint server for biology · 2026Article
- Membrane curvature initiates Cdc42-FBP17-N-WASP clustering and actin nucleation.The EMBO journal · 2026Article
- LamelliQuant: An Automated Analysis Pipeline for Lamellipodia Protrusion Dynamics.Cytoskeleton (Hoboken, N.J.) · 2026Article
- ERK builds a population of short-lived nascent adhesions that produce persistent edge protrusion and cell migration.bioRxiv : the preprint server for biology · 2025Article
- NDR1/2 kinases regulate cell polarization and cell motility through Cdc42 GTPase and Pard3 signaling in mammalian cells.bioRxiv : the preprint server for biology · 2025Article
- Morphological control of merlin-Rac antagonism in proliferation-promoting signaling.Science signaling · 2025Article
- A competition network connects Rab5 and Rab11 GTPases at the surface of endocytic structures.iScience · 2025Article
- Spatiotemporal analysis of F-actin polymerization with micropillar arrays reveals synchronization between adhesion sites.Molecular biology of the cell · 2024Article
- Interpretable Fine-Grained Phenotypes of Subcellular Dynamics via Unsupervised Deep Learning.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Proteolysis-free amoeboid migration of melanoma cells through crowded environments via bleb-driven worrying.Developmental cell · 2024Article
- Compression-dependent microtubule reinforcement enables cells to navigate confined environments.Nature cell biology · 2024Article
- Article
- Filopodial protrusion driven by density-dependent Ena-TOCA-1 interactions.Journal of cell science · 2024Article
- Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.Current biology : CB · 2024Article
- Myosin-independent stiffness sensing by fibroblasts is regulated by the viscoelasticity of flowing actin.Communications materials · 2024Article
- Nascent adhesions shorten the period of lamellipodium protrusion through the Brownian ratchet mechanism.Molecular biology of the cell · 2023Article
- Pulses of RhoA Signaling Stimulate Actin Polymerization and Flow in Protrusions to Drive Collective Cell Migration.bioRxiv : the preprint server for biology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Highly redundant pathways often contain components whose functions are difficult to decipher from the responses induced by genetic or molecular perturbations. Here, we present a statistical approach that samples and registers events observed in images of intrinsic fluctuations in unperturbed cells to establish the functional hierarchy of events in systems with redundant pathways. We apply this approach to study the recruitment of actin assembly factors involved in the protrusion of the cell membrane. We find that the formin mDia1, along with nascent adhesion components, is recruited to the leading edge of the cell before protrusion onset, initiating linear growth of the lamellipodial network. Recruitment of Arp2/3, VASP, cofilin, and the formin mDia2 then promotes sustained exponential growth of the network. Experiments changing membrane tension suggest that Arp2/3 recruitment is mechano-responsive. These results indicate that cells adjust the overlapping contributions of multiple factors to actin filament assembly during protrusion on a ten-second timescale and in response to mechanical cues.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.