Evidence map›Paper›PMID 26273641›Full record

ArticleBioMed research international2015

Translational Control Protein 80 Stimulates IRES-Mediated Translation of p53 mRNA in Response to DNA Damage.

Marie-Jo Halaby, Yan Li, Benjamin R Harris, Shuxia Jiang, W Keith Miskimins, Margot P Cleary, Da-Qing Yang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 26 citations in OpenAlex.

  1. Review
  2. DNA and RNA Binding Proteins: From Motifs to Roles in Cancer.International journal of molecular sciences · 2022
    Review
  3. Review
  4. Article
  5. Review
  6. Review
  7. Article
  8. Review
  9. Review
  10. Translational Control inInternational journal of molecular sciences · 2019
    Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Article
  16. Targeting RNA helicases in cancer: The translation trap.Biochimica et biophysica acta. Reviews on cancer · 2017
    Review
  17. Review
  18. Targeting IRES-Mediated p53 Synthesis for Cancer Diagnosis and Therapeutics.International journal of molecular sciences · 2017
    Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Marie-Jo HalabyThe Hormel Institute, University of Minnesota, Austin, MN 55912, USA.
Yan LiThe Hormel Institute, University of Minnesota, Austin, MN 55912, USA.
Benjamin R HarrisThe Hormel Institute, University of Minnesota, Austin, MN 55912, USA.
Shuxia JiangThe Hormel Institute, University of Minnesota, Austin, MN 55912, USA.
W Keith MiskiminsCancer Biology Research Center, Sanford Research/University of South Dakota, Sioux Falls, SD 57104, USA.
Margot P ClearyThe Hormel Institute, University of Minnesota, Austin, MN 55912, USA ; The Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
Da-Qing YangThe Hormel Institute, University of Minnesota, Austin, MN 55912, USA ; The Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
University of Minnesota · USHormel (United States) · USUniversity of South Dakota · US

Funding

TRANSLATIONAL CONTROL OF P27 IN BREAST CANCER CELLSR01CA084325 · NCI · UNIVERSITY OF SOUTH DAKOTA · PI MISKIMINS, W KEITH · 2000 to 2008
$1.8M
Defective p53 synthesis following DNA damage and cancer developmentR03ES017869 · NIEHS · UNIVERSITY OF MINNESOTA · PI YANG, DA-QING · 2010 to 2011
$155k
Modulation of p53 induction by targeting cap-dependent translation in cancerR03CA177954 · NCI · UNIVERSITY OF MINNESOTA · PI YANG, DA-QING · 2013 to 2014
$150k
NCI NIH HHS 1R01#CA084325NCI NIH HHS 1R03#CA177954NCI NIH HHS BC051719NCI NIH HHS R01 CA084325NCI NIH HHS R03 CA177954NIEHS NIH HHS 1R03#ES017869NIEHS NIH HHS R03 ES017869
6 · The paper itself

Abstract

Synthesis of the p53 tumor suppressor increases following DNA damage. This increase and subsequent activation of p53 are essential for the protection of normal cells against tumorigenesis. We previously discovered an internal ribosome entry site (IRES) that is located at the 5'-untranslated region (UTR) of p53 mRNA and found that the IRES activity increases following DNA damage. However, the mechanism underlying IRES-mediated p53 translation in response to DNA damage is still poorly understood. In this study, we discovered that translational control protein 80 (TCP80) has increased binding to the p53 mRNA in vivo following DNA damage. Overexpression of TCP80 also leads to increased p53 IRES activity in response to DNA damage. TCP80 has increased association with RNA helicase A (RHA) following DNA damage and overexpression of TCP80, along with RHA, leads to enhanced expression of p53. Moreover, we found that MCF-7 breast cancer cells with decreased expression of TCP80 and RHA exhibit defective p53 induction following DNA damage and diminished expression of its downstream target PUMA, a proapoptotic protein. Taken together, our discovery of the function of TCP80 and RHA in regulating p53 IRES and p53 induction following DNA damage provides a better understanding of the mechanisms that regulate IRES-mediated p53 translation in response to genotoxic stress.

Indexed as

DNA DamageHumansInternal Ribosome Entry SitesMCF-7 CellsNuclear Factor 90 ProteinsProtein BiosynthesisRNA, MessengerTumor Suppressor Protein p53ILF3 protein, humanInternal Ribosome Entry SitesNuclear Factor 90 ProteinsRNA, MessengerTumor Suppressor Protein p53

Identifiers

PMID26273641
PMCPMC4529924
OpenAlexW1549787913

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.