ArticleBioMed research international2015
Expression of Heterologous Cellulases in Thermotoga sp. Strain RQ2.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization.Microorganisms · 2023Review
- Adapted laboratory evolution of Thermotoga sp. strain RQ7 under carbon starvation.BMC research notes · 2022Article
- Improvement of COMicroorganisms · 2021Article
- Construction and Validation of a Genome-Scale Metabolic Network of Thermotoga sp. Strain RQ7.Applied biochemistry and biotechnology · 2021Article
- Effect of Cultivation Parameters on Fermentation and Hydrogen Production in the PhylumInternational journal of molecular sciences · 2020Review
- Insights into Thermophilic Plant Biomass Hydrolysis fromMicroorganisms · 2020Review
- Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses.Applied and environmental microbiology · 2018Article
- Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms.Wiley interdisciplinary reviews. Systems biology and medicine · 2017Review
- Development of a pyrE-based selective system for Thermotoga sp. strain RQ7.Extremophiles : life under extreme conditions · 2017Article
- Contribution of Pentose Catabolism to Molecular Hydrogen Formation by Targeted Disruption of Arabinose Isomerase (araA) in the Hyperthermophilic Bacterium Thermotoga maritima.Applied and environmental microbiology · 2017Article
- Complete genome sequence ofStandards in genomic sciences · 2017Article
- Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals.Frontiers in microbiology · 2015Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of Thermotoga spp. to degrade cellulose is limited due to a lack of exoglucanases. To address this deficiency, cellulase genes Csac_1076 (celA) and Csac_1078 (celB) from Caldicellulosiruptor saccharolyticus were cloned into T. sp. strain RQ2 for heterologous overexpression. Coding regions of Csac_1076 and Csac_1078 were fused to the signal peptide of TM1840 (amyA) and TM0070 (xynB), resulting in three chimeric enzymes, namely, TM1840-Csac_1078, TM0070-Csac_1078, and TM0070-Csac_1076, which were carried by Thermotoga-E. coli shuttle vectors pHX02, pHX04, and pHX07, respectively. All three recombinant enzymes were successfully expressed in E. coli DH5α and T. sp. strain RQ2, rendering the hosts with increased endo- and/or exoglucanase activities. In E. coli, the recombinant enzymes were mainly bound to the bacterial cells, whereas in T. sp. strain RQ2, about half of the enzyme activities were observed in the culture supernatants. However, the cellulase activities were lost in T. sp. strain RQ2 after three consecutive transfers. Nevertheless, this is the first time heterologous genes bigger than 1 kb (up to 5.3 kb in this study) have ever been expressed in Thermotoga, demonstrating the feasibility of using engineered Thermotoga spp. for efficient cellulose utilization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.