ArticleScientific reports2015
Artificial biofilms establish the role of matrix interactions in staphylococcal biofilm assembly and disassembly.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- PslG-Mediated Disruption ofInternational journal of molecular sciences · 2026Article
- Temperature-dependent reprogramming of virulence traits duringbioRxiv : the preprint server for biology · 2026Article
- Assessment of Antibiotic Sensitivity in Biofilms Using GelMA Hydrogel Microspheres.Gels (Basel, Switzerland) · 2026Article
- Soft X-ray tomography reveals variations in B. subtilis biofilm structure upon tasA deletion.NPJ biofilms and microbiomes · 2025Article
- The landscape of biofilm models for phage therapy: mimicking biofilms in diabetic foot ulcers using 3D models.Frontiers in microbiology · 2025Review
- Bacteria Colonies Modify Their Shear and Compressive Mechanical Properties in Response to Different Growth Substrates.ACS applied bio materials · 2024Article
- Dissolvable alginate hydrogel-based biofilm microreactors for antibiotic susceptibility assays.Biofilm · 2023Article
- Phenotypic and Genomic Insights into Biofilm Formation in Antibiotic-Resistant Clinical Coagulase-NegativeGenes · 2022Article
- Enterococcal biofilm-A nidus for antibiotic resistance transfer?Journal of applied microbiology · 2022Review
- The structural role of bacterial eDNA in the formation of biofilm streamers.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Detecting Redox Potentials Using Porous Boron Nitride/ATP-DNA Aptamer/Methylene Blue Biosensor to Monitor Microbial Activities.Micromachines · 2022Article
- pH variation in medical implant biofilms: Causes, measurements, and its implications for antibiotic resistance.Frontiers in microbiology · 2022Review
- Capsicumicine, a New Bioinspired Peptide from Red Peppers Prevents Staphylococcal BiofilmMicrobiology spectrum · 2021Article
- A Novel Biofilm Model System to Visualise Conjugal Transfer of Vancomycin Resistance by Environmental Enterococci.Microorganisms · 2021Article
- Biofilm-Formation Ability and the Presence of Adhesion Genes in Coagulase-Negative Staphylococci Isolates from Chicken Broilers.Animals : an open access journal from MDPI · 2021Article
- Searching for the Secret of Stickiness: How Biofilms Adhere to Surfaces.Frontiers in microbiology · 2021Review
- Non-invasive single-cell morphometry in living bacterial biofilms.Nature communications · 2020Article
- Nonlinear rheological characteristics of single species bacterial biofilms.NPJ biofilms and microbiomes · 2020Article
- AC Electric Field Mediated Assembly of Bacterial Tetrads.ACS omega · 2020Article
- Phenotypic and Molecular Detection of Biofilm Formation inPathogens (Basel, Switzerland) · 2020Article
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4 authors.
Funding
Abstract
We demonstrate that the microstructural and mechanical properties of bacterial biofilms can be created through colloidal self-assembly of cells and polymers, and thereby link the complex material properties of biofilms to well understood colloidal and polymeric behaviors. This finding is applied to soften and disassemble staphylococcal biofilms through pH changes. Bacterial biofilms are viscoelastic, structured communities of cells encapsulated in an extracellular polymeric substance (EPS) comprised of polysaccharides, proteins, and DNA. Although the identity and abundance of EPS macromolecules are known, how these matrix materials interact with themselves and bacterial cells to generate biofilm morphology and mechanics is not understood. Here, we find that the colloidal self-assembly of Staphylococcus epidermidis RP62A cells and polysaccharides into viscoelastic biofilms is driven by thermodynamic phase instability of EPS. pH conditions that induce phase instability of chitosan produce artificial S. epidermidis biofilms whose mechanics match natural S. epidermidis biofilms. Furthermore, pH-induced solubilization of the matrix triggers disassembly in both artificial and natural S. epidermidis biofilms. This pH-induced disassembly occurs in biofilms formed by five additional staphylococcal strains, including three clinical isolates. Our findings suggest that colloidal self-assembly of cells and matrix polymers produces biofilm viscoelasticity and that biofilm control strategies can exploit this mechanism.
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