ArticleThe Journal of biological chemistry2015
A Novel Basal Body Protein That Is a Polo-like Kinase Substrate Is Required for Basal Body Segregation and Flagellum Adhesion in Trypanosoma brucei.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Polo-like kinase phosphorylation of the orphan kinesin KIN-G negatively regulates centrin arm biogenesis ineLife · 2026Article
- Distinguishing functions of trypanosomatid protein kinases.Trends in parasitology · 2022Review
- Polo-like kinase and Aurora B kinase phosphorylate and cooperate with the CIF1-CIF2 complex to promote cytokinesis initiation inOpen biology · 2022Article
- Article
- The kinetoplastid-specific phosphatase KPP1 attenuates PLK activity to facilitate flagellum inheritance inScience signaling · 2021Article
- Alternate histories of cytokinesis: lessons from the trypanosomatids.Molecular biology of the cell · 2020Review
- Polo-like kinase in trypanosomes: an odd member out of the Polo family.Open biology · 2020Article
- Regulated protein stabilization underpins the functional interplay among basal body components inThe Journal of biological chemistry · 2020Article
- Organising the cell cycle in the absence of transcriptional control: Dynamic phosphorylation co-ordinates the Trypanosoma brucei cell cycle post-transcriptionally.PLoS pathogens · 2019Article
- BOH1 cooperates with Polo-like kinase to regulate flagellum inheritance and cytokinesis initiation inJournal of cell science · 2019Article
- The trypanosome-specific protein CIF3 cooperates with the CIF1 protein to promote cytokinesis inThe Journal of biological chemistry · 2018Article
- Flagellum inheritance inThe Journal of biological chemistry · 2018Article
- Functional analyses of the CIF1-CIF2 complex in trypanosomes identify the structural motifs required for cytokinesis.Journal of cell science · 2017Article
- Article
- CRL4WDR1 Controls Polo-like Kinase Protein Abundance to Promote Bilobe Duplication, Basal Body Segregation and Flagellum Attachment in Trypanosoma brucei.PLoS pathogens · 2017Article
- An EF-hand-containing Protein in Trypanosoma brucei Regulates Cytokinesis Initiation by Maintaining the Stability of the Cytokinesis Initiation Factor CIF1.The Journal of biological chemistry · 2016Article
- Two distinct cytokinesis pathways drive trypanosome cell division initiation from opposite cell ends.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- SAS-4 Protein in Trypanosoma brucei Controls Life Cycle Transitions by Modulating the Length of the Flagellum Attachment Zone Filament.The Journal of biological chemistry · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The Polo-like kinase (PLK) in Trypanosoma brucei plays multiple roles in basal body segregation, flagellum attachment, and cytokinesis. However, the mechanistic role of TbPLK remains elusive, mainly because most of its substrates are not known. Here, we report a new substrate of TbPLK, SPBB1, and its essential roles in T. brucei. SPBB1 was identified through yeast two-hybrid screening with the kinase-dead TbPLK as the bait. It interacts with TbPLK in vitro and in vivo, and is phosphorylated by TbPLK in vitro. SPBB1 localizes to both the mature basal body and the probasal body throughout the cell cycle, and co-localizes with TbPLK at the basal body during early cell cycle stages. RNAi against SPBB1 in procyclic trypanosomes inhibited basal body segregation, disrupted the new flagellum attachment zone filament, detached the new flagellum, and caused defective cytokinesis. Moreover, RNAi of SPBB1 confined TbPLK at the basal body and the bilobe structure, resulting in constitutive phosphorylation of TbCentrin2 at the bilobe. Altogether, these results identified a basal body protein as a TbPLK substrate and its essential role in promoting basal body segregation and flagellum attachment zone filament assembly for flagellum adhesion and cytokinesis initiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.