ArticleBMC oral health2015
Development of a polymerase chain reaction assay for the rapid detection of the oral pathogenic bacterium, Selenomonas noxia.
Article in BMC oral health, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- The Prevalence of the Periodontal PathogenClinical, cosmetic and investigational dentistry · 2025Article
- Oral Prevalence ofPathogens (Basel, Switzerland) · 2024Article
- Higher Prevalence of the Periodontal PathogenPathogens (Basel, Switzerland) · 2024Article
- Screening forInternational journal of environmental research and public health · 2024Article
- Supragingival microbiome variations and the influence ofJournal of oral microbiology · 2024Article
- Administration of Clinical COVID-19 Mouthwashing Protocol and Potential Modulation of Pediatric Oral Bacterial Prevalence ofPediatric reports · 2023Article
- Processing and storage methods affect oral and gut microbiome composition.Frontiers in microbiology · 2023Article
- Microbial Screening Reveals Oral Site-Specific Locations of the Periodontal PathogenCurrent issues in molecular biology · 2021Article
- Plasmonic and Electrostatic Interactions Enable Uniformly Enhanced Liquid Bacterial Surface-Enhanced Raman Scattering (SERS).Nano letters · 2020Article
- HPV infection and bacterial microbiota in the placenta, uterine cervix and oral mucosa.Scientific reports · 2018Article
- Portable device for the detection of colorimetric assays.Royal Society open science · 2017Article
- Characterization and Discrimination of Gram-Positive Bacteria Using Raman Spectroscopy with the Aid of Principal Component Analysis.Nanomaterials (Basel, Switzerland) · 2017Article
- Key Microbiota Identification Using Functional Gene Analysis during Pepper (Piper nigrum L.) Peeling.PloS one · 2016Article
- Effects of the application of high-pressure oxygen on the treatment of periodontal disease in diabetic patients.ORAL & implantologyArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn recent studies, periodontal health has been linked to being overweight and/or obese. Among common oral bacteria, Selenomonas noxia has been implicated in converting periodontal health to disease, and Selenomonas species have also been found in gastric ulcers. The objective of this study was to develop and validate a quantitative polymerase chain reaction (qPCR) assay for the specific and rapid detection of S. noxia.
methodsTwo oligonucleotide primer pairs and one probe were designed and tested to determine optimal amplification signal with three strains of S. noxia. The PCR assay was tested against fourteen non-target organisms, including closely related oral Selenomonads, one phylogenetically closely related bacterium, and two commonly isolated oral bacteria.
resultsOne of the primer sets was more sensitive at detecting the target organism and was selected for optimization and validation experiments. The designed primers and probe amplified the target organism with 100% specificity. PCR inhibition was observed with an internal positive control, and inhibition was resolved by diluting the DNA extract.
conclusionsThe qPCR assay designed in this study can be used to specifically detect S. noxia in the clinical setting and in future research involving the enhanced detection of S. noxia. The assay can also be used in epidemiological studies for understanding the role of S. noxia in disease processes including, but not limited to, oral health and obesity of infectious origin.
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