Evidence map›Paper›PMID 26272247›Full record

ReviewMethods (San Diego, Calif.)2015

Flavivirus RNA synthesis in vitro.

Radhakrishnan Padmanabhan, Ratree Takhampunya, Tadahisa Teramoto, Kyung H Choi

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Methods (San Diego, Calif.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Radhakrishnan PadmanabhanDepartment of Microbiology and Immunology, Georgetown University School of Medicine, Washington DC 20057, United States. Electronic address: rp55@georgetown.edu.
Ratree TakhampunyaDepartment of Microbiology and Immunology, Georgetown University School of Medicine, Washington DC 20057, United States.
Tadahisa TeramotoDepartment of Microbiology and Immunology, Georgetown University School of Medicine, Washington DC 20057, United States.
Kyung H ChoiDepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, United States.

Funding

MECHANISM OF RNA SYNTHESIS BY DENGUE VIRUS NS5R01AI087856 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI Kyung H Choi · 2011 to 2026
$5.5M
Lethal Mutagenesis as an Antiviral StrategyU01AI054776 · NIAID · PENNSYLVANIA STATE UNIVERSITY-UNIV PARK · PI CAMERON, CRAIG E. · 2003 to 2007
$3.1M
FUNCTIONAL ANALYSIS OF DENGUE VIRUS ANTIGENS NS3 AND NS5R01AI032078 · NIAID · UNIVERSITY OF KANSAS MEDICAL CENTER · PI PADMANABHAN, RADHAKRISHNAN · 1993 to 2003
$1.0M
FUNCTIONAL ANALYSIS OF DENGUE VIRUS ANTIGENS NS3 AND NS5R21AI032078 · NIAID · UNIVERSITY OF KANSAS MEDICAL CENTER · PI PADMANABHAN, RADHAKRISHNAN · 1998 to 1998
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NIAID NIH HHS AI087856NIAID NIH HHS AI32078NIAID NIH HHS AI 54776NIAID NIH HHS R01 AI032078NIAID NIH HHS R01 AI087856NIAID NIH HHS R21 AI032078NIAID NIH HHS U01 AI054776
6 · The paper itself

Abstract

Establishment of in vitro systems to study mechanisms of RNA synthesis for positive strand RNA viruses have been very useful in the past and have shed light on the composition of protein and RNA components, optimum conditions, the nature of the products formed, cis-acting RNA elements and trans-acting protein factors required for efficient synthesis. In this review, we summarize our current understanding regarding the requirements for flavivirus RNA synthesis in vitro. We describe details of reaction conditions, the specificity of template used by either the multi-component membrane-bound viral replicase complex or by purified, recombinant RNA-dependent RNA polymerase. We also discuss future perspectives to extend the boundaries of our knowledge.

Indexed as

FlavivirusNucleic Acid Amplification TechniquesRNA-Dependent RNA PolymeraseRNA, ViralRNA-Dependent RNA PolymeraseRNA, ViralCytoplasmic membrane-bound viral replication complexEndogenous and exogenous viral RNA templatesHeparin trapPolarity of RNA product by RNase H mappingRNA-dependent RNA polymerase from positive strand mosquito-borne FlaviviridaeTemperature-dependent de novo initiation and elongation

Identifiers

PMID26272247
PMCPMC4712636

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.