ArticleRNA (New York, N.Y.)2015
Poly(U) RNA-templated synthesis of AppA.
Article in RNA (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- A small polymerase ribozyme that can synthesize itself and its complementary strand.Science (New York, N.Y.) · 2026Article
- Geochemical influences on nonenzymatic oligomerization of prebiotically relevant cyclic nucleotides.RNA (New York, N.Y.) · 2020Article
- Template-Directed Catalysis of a Multistep Reaction Pathway for Nonenzymatic RNA Primer Extension.Biochemistry · 2019Article
- Alarmones as Vestiges of a Bygone RNA World.Journal of molecular evolution · 2019Article
- Eighty routes to a ribonucleotide world; dispersion and stringency in the decisive selection.RNA (New York, N.Y.) · 2018Article
- Non-Watson-Crick RNA synthesis suited to origin functions.RNA (New York, N.Y.) · 2018Article
- A Kinetic Model of Nonenzymatic RNA Polymerization by Cytidine-5'-phosphoro-2-aminoimidazolide.Biochemistry · 2017Article
- Efficient Heritable Gene Expression Readily Evolves in RNA Pools.Journal of molecular evolution · 2017Article
- Biochemical Refinement Before Genetics: Chance Utility.Journal of molecular evolution · 2016Article
- A Highly Reactive Imidazolium-Bridged Dinucleotide Intermediate in Nonenzymatic RNA Primer Extension.Journal of the American Chemical Society · 2016Article
- Cross-backbone templating; ribodinucleotides made on poly(C).RNA (New York, N.Y.) · 2016Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simple nucleotide templating activities are of interest as potential primordial reactions. Here we describe the acceleration of 5'-5' AppA synthesis by 3'-5' poly(U) under normal solution conditions. This reaction is apparently templated via complementary U:A base-pairing, despite the involvement of two different RNA backbones, because poly(U), unlike other polymers, significantly stimulates AppA synthesis. These interactions occur in moderate (K(+)) and (Mg(2+)) and are temperature sensitive, being more efficient at 10°C than at 4°C, but absent at 20°C. The reaction is only slightly pH sensitive, despite potentially relevant substrate pKa's. Kinetic data explicitly support production of AppA by interaction of stacked 2MeImpA and pA nucleotides paired with a single molecule of U template. At a lower rate, AppA can also be produced by a chemical reaction between 2MeImpA and pA, without participation of poly(U). Molecular modeling suggests that 5'-5' joining between stacked or concurrently paired A's can occur without major departures from normal U-A helical coordinates. So, coenzyme-like 5'-5' purine dinucleotides might be readily synthesized from 3'-5' RNAs with complementary sequences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.