ArticleMethods in molecular biology (Clifton, N.J.)2015
Measurement of Apoptotic and Necrotic Cell Death in Primary Hepatocyte Cultures.
Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed.
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- Large-Scale Expansion of Human Liver Stem Cells Using Two Different Bioreactor Systems.Bioengineering (Basel, Switzerland) · 2024Article
- Modifications in cellular viability, DNA damage and stress responses inflicted in cancer cells by copper-64 ions.Frontiers in medicine · 2023Article
- Commensal bacteria of the lung microbiota synergistically inhibit inflammation in a three-dimensional epithelial cell model.Frontiers in immunology · 2023Article
- Chemistry and Bioactivity ofMolecules (Basel, Switzerland) · 2022Article
- Effects of Drugs Formerly Suggested for COVID-19 Repurposing on Pannexin1 Channels.International journal of molecular sciences · 2022Article
- Effect of microplastics on Yersinia ruckeri infection in rainbow trout (Oncorhynchus mykiss).Environmental science and pollution research international · 2022Article
- Population balance modelling captures host cell protein dynamics in CHO cell cultures.PloS one · 2022Article
- Autophagy deficiency exacerbates acute lung injury induced by copper oxide nanoparticles.Journal of nanobiotechnology · 2021Article
- In Vitro Liver Toxicity Testing of Chemicals: A Pragmatic Approach.International journal of molecular sciences · 2021Review
- CaCancers · 2021Review
- Flavonoid-rich fraction attenuates permethrin-induced toxicity by modulating ROS-mediated hepatic oxidative stress and mitochondrial dysfunction ex vivo and in vivo in rat.Environmental science and pollution research international · 2021Article
- Effects of microplastic exposure on the blood biochemical parameters in the pond turtle (Emys orbicularis).Environmental science and pollution research international · 2021Article
- Identification of a novel subpopulation of Caspase-4 positive non-small cell lung Cancer patients.Journal of experimental & clinical cancer research : CR · 2020Article
- Antitumor Activity of Ruthenium(II) Terpyridine Complexes towards Colon Cancer Cells In Vitro and In Vivo.Molecules (Basel, Switzerland) · 2020Article
- Excessive neutrophil levels in the lung underlie the age-associated increase in influenza mortality.Mucosal immunology · 2019Article
- Anticancer Activity of the Goat Antimicrobial Peptide ChMAP-28.Frontiers in pharmacology · 2018Article
- Cytotoxic and Genotoxic Effects of Fluconazole on African Green Monkey Kidney (Vero) Cell Line.BioMed research international · 2018Article
- Experimental and clinical evidence of differential effects of magnesium sulfate on neuroprotection and angiogenesis in the fetal brain.Pharmacology research & perspectives · 2017Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Hepatotoxicity, including drug-induced liver injury, is frequently accompanied by cell death. The latter is typically driven by apoptosis or necrosis, which substantially differ based upon biochemical and morphological criteria. This chapter describes two commonly used methods to probe apoptotic and necrotic activities in adherent monolayer cultures of primary hepatocytes. The apoptosis assay uses a prototypical substrate of caspase 3, the main executor of apoptotic cell death, which can be cleaved, yielding a product that can be measured fluorimetrically. The second assay relies on the disruption of the cell plasma membrane, which typically occurs in necrotic cell death and that results in the extracellular release of cytoplasmic enzymes, such as lactate dehydrogenase. The latter can be indirectly assessed by spectrophotometrically measuring the consumption of reduced nicotinamide adenine dinucleotide.
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Registered trials
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