Evidence map›Paper›PMID 26271617›Full record

ArticleLipids2015

Fucoxanthin Enhances Chain Elongation and Desaturation of Alpha-Linolenic Acid in HepG2 Cells.

Meng-Ting Wu, Hui-Min Su, Yi Cui, Anthony Windust, Hong-Nong Chou, Ching-Jang Huang

Abstract read
PubMed Publisher
In one paragraph

Article in Lipids, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 12 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

Meng-Ting WuDepartment of Biochemical Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan.
Hui-Min SuInstitute of Physiology, College of Medicine, National Taiwan University, 1, Sec. 1, Jen-Ai Rd., Taipei, 10051, Taiwan.
Yi CuiMeasurement Science and Standards, National Research Council, 1200 Montreal Road Building M-12, Room B-12B, Ottawa, ON, K1A 0R6, Canada.
Anthony WindustMeasurement Science and Standards, National Research Council, 1200 Montreal Road Building M-12, Room B-12B, Ottawa, ON, K1A 0R6, Canada.
Hong-Nong ChouInstitute of Fisheries Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan.
Ching-Jang HuangDepartment of Biochemical Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan. cjjhuang@ntu.edu.tw.
National Taiwan University · TWNational Research Council Canada · CAWeatherford College · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dietary fucoxanthin (FX), a carotenoid compound from brown algae, was found to increase docosahexaenoic acid (DHA, 22:6n-3) and arachidonic acid (ARA, 20:4n-6) in the liver of mice. DHA and ARA are known to be biosynthesized from the respective precursor α-linolenic acid (ALA, 18:3n-3) and linoleic acid (LNA, 18:2n-6), through desaturation and chain elongation. We examined the effect of FX on the fatty acid metabolism in HepG2 cells (Hepatocellular carcinoma, human). In the first experiment, cells were co-treated with ALA (100 μM) and FX (0-100 μM) or vehicle for 48 h. FX increased eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3), DHA at concentrations of ≥ 50 μM. To clarify the change in the metabolism of polyunsaturated fatty acid (PUFA), in the second experiment, cells were co-treated with universally-[(13)C]-labeled (U-[(13)C]-) ALA (100 μM) and FX (100 μM) for 0.5, 3, 6, 24 and 48 h. [(13)C] labeled-EPA, DPA and DHA content in HepG2 cells were all increased by FX after 48 h treatment. Furthermore, estimated delta-5 desaturase (D5D) but not delta-6 desaturase (D6D) activity index was increased at 48 h. These results suggested that FX may enhance the conversion of ALA to longer chain n-3 PUFA through increasing D5D activity in the liver.

Indexed as

alpha-Linolenic AcidDelta-5 Fatty Acid DesaturaseEicosapentaenoic AcidFatty Acid DesaturasesFatty Acids, UnsaturatedGene Expression Regulation, EnzymologicHep G2 CellsHumansXanthophyllsalpha-Linolenic AcidDelta-5 Fatty Acid Desaturasedocosapentaenoic acidEicosapentaenoic AcidFADS2 protein, humanFatty Acid DesaturasesFatty Acids, UnsaturatedfucoxanthinXanthophyllsALADelta-5 desaturaseDHAElongationEPAFucoxanthinn-3 PUFA

Identifiers

PMID26271617
OpenAlexW1144794890

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.