ArticleScientific reports2015
TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- In vivo genome-wide CRISPR screening identifies CITED2 as a driver of prostate cancer bone metastasis.Oncogene · 2024Article
- Comparison of ex vivo bioluminescence imaging, Alu-qPCR and histology for the quantification of spontaneous lung and bone metastases in subcutaneous xenograft mouse models.Clinical & experimental metastasis · 2024Article
- Interspecies chimerism with human embryonic stem cells generates functional human dopamine neurons at low efficiency.Stem cell reports · 2024Article
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- Unchecked oxidative stress in skeletal muscle prevents outgrowth of disseminated tumour cells.Nature cell biology · 2022Article
- PCR-Based Analytical Methods for Quantification and Quality Control of Recombinant Adeno-Associated Viral Vector Preparations.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Biodistribution studies for cell therapy products: Current status and issues.Regenerative therapy · 2021Review
- Article
- DNA Extraction with TRIzol Reagent Using a Silica Column.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning.Cell reports · 2021Article
- Human-specific GAPDH qRT-PCR is an accurate and sensitive method of xenograft metastasis quantification.Molecular therapy. Methods & clinical development · 2021Article
- Osteocyte Vegf-a contributes to myeloma-associated angiogenesis and is regulated by Fgf23.Scientific reports · 2020Article
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- Investigating circulating tumor cells and distant metastases in patient-derived orthotopic xenograft models of triple-negative breast cancer.Breast cancer research : BCR · 2019Article
- Interleukin-7 Contributes to the Invasiveness of Prostate Cancer Cells by Promoting Epithelial-Mesenchymal Transition.Scientific reports · 2019Article
- Down-regulation of FZD3 receptor suppresses growth and metastasis of human melanoma independently of canonical WNT signaling.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Characterization of prostate cancer cell progression in zebrafish xenograft model.International journal of oncology · 2018Article
- Highly sensitive and specific Alu-based quantification of human cells among rodent cells.Scientific reports · 2017Article
- Skeletal Colonization by Breast Cancer Cells Is Stimulated by an Osteoblast and β2AR-Dependent Neo-Angiogenic Switch.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2017Article
- Safety of Intraovarian Injection of Human Mesenchymal Stem Cells in a Premature Ovarian Insufficiency Mouse Model.Cell transplantationArticle
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu sequence qPCR amplification. This approach enables precise quantification of tumor cells and corresponding host gene expression during metastatic colonization in xenograft models.
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Registered trials
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